阴离子化高分子包覆非病毒载体提高有血清环境下RNA干扰效率的研究

doi:10.3969/j.issn.0258-8021. 2016. 04.008

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Abstract The clinical application of cationic polymer carriers was limited because the adsorption of protein in serum environment led to low transfection efficiency. In this work, we synthesized carboxylic Dextran (Dex-COOH) and water-soluble carboxylic fullerene (C60-Dex-COOH) anionic polymers, coated on the pullulan-spermine and siRNA complex (PS/siRNA) to reduce the adsorption of serum protein. The particle size and Zeta potential were analyzed by DLS/ELS, respectively. The formation of anion coating and bovine serum albumin (BSA) adsorption of complexes were tested by QCM-D. Cellular uptake, cytotoxicity on the Hela cells and RNAi efficiency on the Hela-EGFP cells were examined by cck-8 reagent and flow cytometry. The results showed that Dex-COOH and C60-Dex-COOH could coat on PS/siRNA to form stable electronegative complexes, prevent the adsorption of serum effectively. Cytotoxicity of complexes was reduced in the absence of serum. On the other hand, in the serum environment, anionic polymer coated complexes could help complexes enter cells and PS/siRNA@C60-Dex-COOH with visible light irradiation would escape from the lysosome, increased the interference efficiency by 20% compared with PS/siRNA. In summary, this coating strategy provided an effective solution of increasing RNAi efficiency mediated by cationic polymers.

Key words： cationic polymer siRNA carrier anionic coating protein adsorption RNA interference

Received: 21 March 2016

PACS:

R318

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	Articles by authors
	Wang Jing
	Xie Lifei
	Meng Jie
	Liu Jian
	Xu Haiyan

Cite this article:

Wang Jing,Xie Lifei,Meng Jie, et al. Anionic Polymer Coating Enhance the RNAi Efficiency of Non-Viral Carriers in Serum Containing Environment[J]. Chinese Journal of Biomedical Engineering, 2016, 35(4): 445-452.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021. 2016. 04.008 OR http://cjbme.csbme.org/EN/Y2016/V35/I4/445

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