Biodegradation of Electrospun Poly (Llactic acidcoεcaprolactone)/Collagen Composite Vascular Grafts
1 Department of Vascular Surgery, Sixth People’s Hospital of Shanghai, Shanghai 200233, China
2 Biomaterials and Tissue Engineering Laboratory, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
Abstract To evaluate the biodegradation and biocompatibility of poly (Llactic acidcoεcaprolactone) (P(LLACL))/collagen graft, P (LLACL) collagen graft scaffolds were made through electrospinning. The prepared scaffolds were set as experimental group (n=40) and the commercial artificial vessel made
of ePTFE was set as control group (n=40). The scaffold samples were implanted in the left dorsal muscle of rabbits, while ePTFE graft materials were implanted in the opposite side. On day 10, 30, 90 and 180 after the implantation, the samples were harvested. The histological staining was applied to observe the degradation of the material and tissue reaction. The number of neutrophils, macrophages and lymphocytes were counted at the high magnification (400×) using the optical microscope. Under the microscope, slight tissue reaction was revealed in the area of P (LLACL)/collagen material. On day 90, the material broken was observed. Some connective fibrous tissue was revealed in the material, a capsulelike membrane was established over the area. During the process of degradation, most of the materials were absorbed within 180 days. The structure and distribution of the tissue cells was highly similar to that of the surrounding area, indicating that the remodeling of the embedded tissue was almost finished. The ePTFE materials were revealed still maintaining the structure encompassed in a thin layer of fibrous connective tissue. The result of cell count showed that the type and trend of cell reaction had no difference after the different stages of postoperation. However, the count of neutrophile granulocyte in experimental group was more than that in the control group on day 10 (2244±372 vs 1922±181 /025 mm2, P<005), while no difference of various types of cells was observed between the two groups post operation. The degeneration of P (LLACL)/collagen material was 90-180 days, which is a satisfying biological performance in the degradation and biocompatibility.
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