|
|
|
| An Inducible Bone Repair Composite Material PHBV/CS: The Renovation of Rabbit Radius |
| Li Gen Zhen Ping Cheng Manping Zhao Hongbin* |
| Lanzhou General Hospital of Lanzhou Command, Lanzhou 730050, China |
|
|
|
|
Abstract In this work, four kinds of chitosan/poly hydroxybutyrate-co-hydroxyvalerate(PHBV)composite scaffold were fabricated by the method of two phase mixture freeze drying.The repair effects of the scaffoldswere investigated and compared to determine the optimal formula for guiding bone regeneration as well as drug releasing. The icariin (Ica) was used in this study as an osteoinductive factor, and Ica microspheres were fabricated by W/O method and covalent binding. Microstructures of the scaffolds and drug releasing behaviors were investigated.After that,the scaffolds were implanted in the radius defects of rabbits;X-ray and 3D CT were applied to observe the repair effect at 1, 3, and 6 months post surgery. The osteogenesis inducible effects were evaluated using HE and Masson staining. The scaffolds showed network-like microstructures with particles inside, the drug loaded particles was 3-11 μm in diameter. The scaffolds released Ica well, though the peak of drug release curve for the covalent binding group was delayed than the others. The peak appeared at 72 h after immersion and quickly reached a stabled level of 75 μg. The images of X-ray and 3-D CT showed the defect of the rabbit radius had connected and the bone mineral density was higher than the other three groups. HE and Masson stained sections of the radius segmental defect of rabbits implanted for 1, 3, and 6 months showed that the scaffold with covalently binding Ica had better repair effect than the others.
|
|
Received: 03 December 2015
|
|
|
|
|
|
[1] Wu Shuilin, Liu Xiangmei, Kelvin WK, et al. Biomimetic porous scaffolds for bone tissue engineering[J]. Materials Science and Engineering, 201), 80: 1-36.
[2] Calori GM, Mazza E, Colombo MC. The use of bone-graft substitutes in large bone defects: Any specific needs [J]. Injury, 2011, 42: S56-S63.
[3] 胡金龙,王静成,颜连启, 等. 组织工程学技术治疗骨缺损的最新研究进展 [J]. 中国矫形外科杂志, 2013, 21(2): 150-152.
[4] Kobayashi M, Toguchida J, Oka M. Development of polyvinyl alcohol-hydrogel (PVA-H) shields with a high water content for tendon injury repair [J]. Hand Surg Br, 2001, 26(5):436-440.
[5] Teh TK, Goh JC, Toh SL. Comparative study of random and aliged nanofibrous scaffolds for tendon/ligament tissue engineering [J]. Biomech, 2008, 41(Suppl 1): S527
[6] Khoasteh A, Behnia H, Naghdi N, et al. Effect of different growth factors and carriers on bone regeneration: a systematic review [J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2013,116(6):e405-e423.
[7] Xiao Q, Chen A, Guo F. Effects of Icariin on expression of OPN mRNA and type I collagen in rat osteoblasts in vitro [J]. Huazhong Univ Sci Technolog Med Sci, 2005, 25(6):690-692.
[8] Heydari A, Khaksar S, TajbakhshM. 1,1,1,3,3,3－Hexafluoroisopropanol: A recyclable organ ocatalyst for N－ BOC protection of amines [J]. Synthesis, 2008, 19: 3126-3130.
[9] 王伟,李文峰,杨玉琼, 等. 缩合剂 1, 3\|二环己基碳二亚胺(DCC ) 在有机合成中的应用 [J]. 化学试剂, 2008,30(3): 185-190, 193.
[10] Tarikere LG, Narayanan R, Michael JL. Solid-phase synthesis of human mucin-derived O-linked Gly copepides [J]. Letters Inpeptide Science, 1996, 3: 79-88.
[11] Wissink MJ, Beernink R, Pieper JS, et al. Immobilization of heparin to EDC/NHS- crosslinked collagen. Characterization and in vitro evaluation [J]. Biomaterials, 2001, 22:151-163.
[12] 费强,尚龙安,范代娣,等. 新型生物降解材料PHBV 动物体内的组织相容性 [J]. 第二军医大学学报, 2009, 30 (2): 220-223.
[13] Miao L, Qiu Z, Yang W, et al. Fully biodegradable poly (3-hydroxybutyrate-co-hydroxyvalerate)/poly(ethylene succinate) blends: Phase behavior, crystallization and mechanical properties [J]. Reactive and Functional Polymers, 2008, 68(2):446-457.
[14] Kose GT, Korkusuz P. Bone generation on PHBV matrices: an in vitro study [J]. Biomaterials, 2003, 24: 4999- 5007
[15] 宋雪莲,于静涛,刘扬,等. 壳聚糖在骨组织工程中应用研究进展 [J]. 中国实用口腔科杂志,2013,6(5):306-310.
[16] Richert L, Lavalle P, Payan E, et al. Layer by layer buildup of polysaccharide films: physical chemistry and cellular adhesion aspects [J]. Langmuir, 2003, 20: 448-458.
[17] Yin YJ, Yao KD, Cheng GX, et al. Properties of polyelectrolyte complex films of chitosan and gelatin [J]. Polym Int, 1999, 48: 429-432.
[18] Nayak TR, Jian L, Phua LC, et al. Thin films of functionalized multiwalled carbon nanotubes as suitable scaffold materials for stem cells proliferation and bone formation [J]. ACS Nano, 2010, 4: 7717-7725.
[19] Massumi M, Abasi M, Babaloo H, et al. The effect of topography on differentiation fates of matrigel-coated mouse embryonic stem cells cultured on PLGA nanofibrous scaffolds [J]. Tissue Eng Part A, 2012, 18: 609-620.
[20] Rambhia KL, Ma P. Controlled drug release for tissue engineering [J]. Journal of Controlled Release, 2015, 219:119-128.
[21] Re′sem T, Kaminer-Israeli Y, Ruvinov E, et al. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds [J]. Biomaterials, 2012, 33(3): 751-761.
[22] Fan H, Tao H, Wu Y, et al. TGF-β3 immobilized PLGA-gelatin/chondroitin sulfate/hyaluronic acid hybrid scaffold for cartilage regeneration [J]. Biomed Mater Res, 2010, 95(4): 982-992.
[23] 贾亮亮,袁丁,王洪武, 等. 淫羊藿苷药理作用的研究进展 [J]. 现代生物医学进展, 2010,10(20):3976-3979.
[24] 于波,杨久山,刘岩, 等. 淫羊藿苷对人成骨细胞的作用[J]. 中医正骨, 2006,18(6):17-19. |
|
|
|
