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| Preparation of Collagen/Silk Fibroin Bicomponent Micro-Nanofibers and its Cytocompatibility Study |
1 Department of Biomedical Engineering, Tianjin Medical University, Tianjin 300070, China
2 Institute of Urological Surgery，Tianjin 300211，China
3 Key Laboratory of Bioactive Materials, Ministry of Education, Tianjin 300071, China |
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Abstract This study aimed to prepare collagen/silk fibroin composite micronanofibers using electrospinning technique, and to evaluate their properties and cytocompatibility. Taking 1,1,1,3,3,3-hexafluoro- 2-propanol(HFIP) as solvent, the composite fibers were prepared according to the mass ratio of collagen/silk fibroin as 100:0.70:30.50:50.30:70.0:100, and then crosslinked by glutaraldehyde (GTA) vapor for 12 h. The fibers were characterized by combined techniques of scanning electron microscopy (SEM), Fourier-transform infrared spectra (FTIR), X-ray diffraction (XRD), thermal analysis and tensile measurements. The composite fibers were inoculated with fibroblasts (3T3). The cell adhesion and proliferation were evaluated by SEM and MTT. The results showed the diameter of composite fibers ranged from 550 nm to 1100 nm, and fiber diameter was positively correlated with SF content. After crosslinking, the β-sheet structure, crystallinity and thermal stability of the nanofibers were improved. This improvement was more obvious with the SF content increases. The mechanical properties of cross-linked fibers were better than those of uncrosslinked fibers, and the best average ultimate tensile strength was (8.70±1.05) MPa when SF content was 70%. The cells grew well on the surface of materials, and had the best adhesion and proliferation on the composite fibers with SF content of 70%, which might be a potential candidate for tissue engineering scaffold.
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