Study on Induction of Human Muse Cells into Neural Precursor Cells in vitro
1 Department of Histology and Embryology, School of Medicine, Nantong University, Nantong 226001, Jiangsu, China
2 Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China
Abstract：The work explored approaches of inducing Muse cells derived from the adult bone marrow into neural precursor cells in vitro. Human bone marrow stromal cells (hBMSCs) were separated and cultured from the healthy adult bone marrow. Then Muse cells were collected using the flow cytometry sorting. In order to observe the characteristics of the pluripotent stem cells, Muse cells were analyzed using immunocytochemical staining and qPCR assay. Afterward, neural inducing culture media was used to induce Muse cells into neural precursor cells (Muse-NPCs). The immunocytochemical staining and qPCR assay were used to observe the expression of the markers specific for the neural stem cell in Muse cells. Muse cells accounting for about 0.58% of hBMSCs were obtained that expressing the markers of the pluripotent stem cells: SSEA-3, Nanog and Oct4. Muse cells also showed significantly higher levels of mRNA of these markers comparing to hBMSCs (P＜0.01). The induced Muse-NPCs expressed the markers of neural stem cells including Nestin and βIII-tubulin. Additionally, the levels of mRNA of these markers in Muse-NPCs was significantly higher than those in hBMSCs and Muse cells (P＜0.01). In conclusion, Muse cells were successfully separated from adult bone marrow and then induced into Muse-NPCs in vitro, providing a new type of seed cells for the cell therapies in the nervous system.
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