Abstract：Nanocarriers may selectively accumulate in tumor tissues through enhanced permeability and retention effect (EPR) or actively targeting functions, and thereby improve the therapeutic efficacy. A range of physicochemical parameters including the size, shape, surface charge, and ligands attachment have been found influencing penetration of nanocarriers in the tumor tissue. Compared with two-dimensional (2D) monolayer cell cultures, three-dimensional (3D) tumor spheroids can better mimic the characteristics of the natural tumor tissues, and therefore have been widely used in the evaluation of tumor penetration and therapeutic efficacy of nanocarriers in recent years. In this review, we will introduce the properties and the preparation methods of 3D tumor spheroids, summarize recent studies investigating the interaction between nanoparticles and 3D tumor spheroids, and discuss the factors that affect the penetration behaviors, therapeutic effects and the intrinsic toxicity of nanocarriers.
于翀立, 张裕英. 三维肿瘤细胞模型在纳米载体性能评价中的应用[J]. 中国生物医学工程学报, 2023, 42(1): 99-109.
Yu Chongli, Zhang Yuying. Application of 3D Tumor Spheroids in Evaluation of Nanocarriers. Chinese Journal of Biomedical Engineering, 2023, 42(1): 99-109.
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