Response of Neuronal Population to High Frequency Electrical Stimulation in Rat Hippocampus
College of Biomedical Engineering and Instrumentation Science, Key Laboratory of Biomedical Engineering of National Education Ministry, Zhejiang University, Hangzhou 310027, China
Abstract：Deep brain stimulations (DBS) have been developed for treating brain diseases such as Parkinson’s disease and epilepsy. But the mechanisms underlying DBS are not clear yet. In order to study the effects of DBS on the neural networks of brain, both orthodromic and antidromichigh frequency stimulation (HFS) were respectively applied on afferent fibers and efferent fibers of the hippocampal CA1 region in anaesthetized rats. Synchronized firings of neuronal action potential (i.e., population spike, PS) of CA1 pyramidal cells were examined. Results showed that 50 Hz orthodromicHFS induced persistent PS firings, while 100 and 200 Hz orthodromicHFS induced much less PS firings. During antidromicHFS with different frequencies, every stimulation pulse evoked a PS. However, the PS amplitude decreased gradually. The decrease of PS amplitude was more rapid with higher stimulation frequency. Taken together, the HFS of axons has excitatory effects on the neuronal populations both upstream and downstream. But the higher the stimulation frequency is, the less the excitatory effect is. These results are important for the further understanding of DBS mechanisms and for the safe and effective application of DBS in clinic.
封洲燕*余颖 曹嘉悦 郭哲杉. 大鼠海马神经元群体对于高频电刺激的响应[J]. 中国生物医学工程学报, 2014, 33(2): 186-193.
FENG Zhou Yan* YU Ying CAO Jia Yue GUO Zhe Shan. Response of Neuronal Population to High Frequency Electrical Stimulation in Rat Hippocampus. journal1, 2014, 33(2): 186-193.
［1］Hamani C, Andrade D, Hodaie M, et al. Deep brain stimulation for the treatment of epilepsy ［J］. Int J Neural Syst, 2009, 19(3): 213-226.
［2］Kuhn J, Mller M, Müller U, et al. Deep brain stimulation for the treatment of addiction ［J］. Addiction, 2011, 106(8): 1536-1537.
［3］Mayberg HS, Lozano AM, Voon V, et al. Deep brain stimulation for treatmentresistant depression ［J］. Neuron, 2005, 45(5): 651-660.
［4］Boon P, Vonck K, De Herdt V, et al. Deep brain stimulation in patients with refractory temporal lobe epilepsy［J］. Epilepsia, 2007, 48(8): 1551-1560.
［5］Jobst B. Brain stimulation for surgical epilepsy［J］. Epilepsy Res, 2010, 89(1): 154-161.
［6］Bragin A, Penttonen M, Buzsaki G. Termination of epileptic afterdischarge in the hippocampus ［J］. J Neurosci, 1997, 17(7): 2567-2579.
［7］Lee HW, Webber WR, Crone N, et al. When is electrical cortical stimulation more likely to produce afterdischarges［J］? Clin Neurophysiol, 2010, 121(1):14-20.
［8］Gwinn RP, Spencer DD. Fighting fire with fire: brain stimulation for the treatment of epilepsy ［J］. Clin Neurosci Res, 2004, 4(1): 95-105.
［9］Deniau JM, Degos B, Bosch C, et al. Deep brain stimulation mechanisms: beyond the concept of local functional inhibition［J］. Eur J Neurosci, 2010, 32(7): 1080-1091.
［10］Feng Zhouyan, Zheng Xiaojing, Yu Ying, et al. Functional disconnection of axonal fibers generated by high frequency stimulation in the hippocampal CA1 region invivo［J］. Brain Res, 2013, 1509: 32-42.
［11］封洲燕, 肖乾江, 胡振华. 电刺激期间神经细胞单元锋电位的检测［J］. 中国生物医学工程学报, 2013, 32(4):403-410.
［12］封洲燕, 邢昊昱, 田聪, 等. 大鼠海马 CA1 区前馈抑制和反馈抑制的作用特性［J］. 航天医学与医学工程, 2011, 24(3): 167-172.
［13］McCarren M, Alger BE. Usedependent depression of IPSPs in rat hippocampal pyramidal cells in vitro［J］. J Neurophysiol, 1985, 53(2): 557-571.
［14］Pearce RA, Grunder SD, Faucher LD. Different mechanisms for usedependent depression of two GABAAmediated IPSCs in rat hippocampus［J］. J Physiol, 1995, 484(Pt 2): 425-435.
［15］Théorêt Y, Brown A, Fleming SP, et al. Hippocampal field potential: a microcomputer aided comparison of amplitude and integral［J］. Brain Res Bull, 1984, 12(5): 589-595.［16］Freund T, Buzsaki G. Interneurons of the hippocampus［J］. Hippocampus, 1996, 6(4): 347-470.
［17］Margineanu DG, Wulfert E. Differential pairedpulse effects of gabazine and bicuculline in rat hippocampal CA3 area［J］. Brain Res Bull, 2000, 51(1): 69-74.
［18］Leung LS, Peloquin P, Canning KJ. Pairedpulse depression of excitatory postsynaptic current sinks in hippocampal CA1 in vivo ［J］. Hippocampus, 2008, 18(10): 1008-1020.
［19］Davies CH, Davies SN, Collingridge GL. Pairedpulse depression of monosynaptic GABAmediated inhibitory postsynaptic responses in rat hippocampus［J］. J Physiol, 1990, 424: 513-531.
［20］Jensen AL, Durand DM. High frequency stimulation can block axonal conduction［J］. Exp Neurol, 2009, 220(1): 57-70.
［21］Zheng Fang, Lammert K, NixdorfBergweiler BE, et al. Axonal failure during high frequency stimulation of rat subthalamic nucleus［J］. J Physiol, 2011, 589(Pt 11): 2781-2793.
［22］Kim E, Owen B, Holmes WR, et al. Decreased afferent excitability contributes to synaptic depression during highfrequency stimulation in hippocampal area CA1［J］. J Neurophysiol, 2012, 108(7): 1965-1976.
［23］Ratas I, Pyragas K. Effect of highfrequency stimulation on nerve pulse propagation in the FitzHugh-Nagumo model［J］. Nonlinear Dyn, 2012, 67(4): 2899-2908.
［24］Sceniak MP, MacIver MB. Cellular actions of urethane on rat visual cortical neurons in vitro［J］. J Neurophysiol, 2006, 95(6): 3865-3874.
