1(School of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453003, Henan, China) 2(Research Center of Neurosense and Control Engineering, Henan Province, Xinxiang 453003, Henan, China) 3(The Second Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, Henan, China)
Abstract：Delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) is a common pathological change in hippocampus, but the pathological process and mechanism in this area during the false recovery period are still unclear. Therefore, this study monitored the long-term local field potential activity in hippocampus to explore the dynamic changes of electroencephalogram signals in this area during DEACMP false recovery. Thirty male SD rats were injected intraperitoneally to make ACMP rat model, electrodes were implanted into the right hippocampus of rats in batches 1～8 weeks after modeling, and local field potential signals of hippocampus of 4～5 rats were collected every week. Local field potential signals of hippocampus of 6 normal rats were used as reference standard before modeling, and the RMS value and energy ratio of α/β/θ/δ wave were extracted by wavelet packet decomposition. Compared with that of before modeling, the amplitude of α/β/θ/δ increased significantly in the early (1/2 week) and late (7/8 week) after modeling (P<0.05), and the root mean square values of four oscillation components showed a changing tendency of increasing first, then decreasing and then increasing from 1 to 8 weeks after modeling. Among them, the δ wave amplitude before modeling was (228±103) μV, and in the 1 and 2 weeks after modeling, it increased to (4 751±4 079) μV and (1 616±1 708) μV. At the 7 and 8 weeks after modeling, the value increased to(1 221±567) μV and (3 222±2 052) μV. The ratio of δ wave energy in the model group from 1 to 8 weeks increased significantly (P<0.05), among them, the δ wave energy proportion before modeling was 0.50±0.09, and increased to 0.62±0.14, 0.65±0.12, 0.77±0.13, 0.73±0.12, 0.59±0.11, 0.70±0.11, 0.71±0.11 and 0.89±0.08 after modeling 1～8 weeks, respectively. In the middle and late stage (5～8 weeks), the δ wave (α wave) gradually increased (decreased) with the progression of the disease (P<0.05). Before modeling the α wave energy ratio was 0.10±0.04, and after modeling (5～8 weeks) it decreased to 0.08±0.02, 0.06±0.03, 0.05±0.02 and 0.01±0.01, respectively. The RMS values of the four kinds of electroencephalogram oscillations in the hippocampus of rats generally increased the risk of DEACMP in the early stage of carbon monoxide poisoning. After poisoning, the energy proportion of δ wave increased significantly throughout the poisoning, and the energy proportion of δ wave and α wave changed unidirectional in the middle and later stages of poisoning. The study provided a new method for the quantitative prediction and evaluation of DEACMP.
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