Abstract：Ultrasound stimulation has neuromodulatory function. In the present study, we explored the neuromodulatory effect and mechanisms of ultrasound stimulation on prefrontal cortex. Ultrasound stimuli were applied transcranially to the prefrontal cortex of anesthetized rats (n=15) and electrocorticogram (ECoG) was recorded before, during (20 min) and after the ultrasound stimulation. The characteristic parameters of ECoG included the total power spectral density (PSD), the PSD of four frequency bands (δ: 0.5～4 Hz, θ: 4～8 Hz, α: 8～13 Hz, β: 13～30 Hz), the average amplitude of ECoG and the average amplitude of the four frequency bands. To attenuate individual differences between experimental rats and reveal the time-effect relationship of ultrasound stimulation, the characteristic parameter values of ECoG during and after ultrasound stimulation were statistically compared and analyzed in the form of percentages relative to pre-stimulation (defined as 100%). The results could be described from four aspects. 1) The whole ultrasound stimulation process was divided into 7 time periods, which were 0~1, 1~2, 2~3, 3~4, 4~5, 5~10,10~20 min of ultrasound stimulation, respectively. The total PSD of ECoG was 139.2±13.2% in prefrontal cortex within 1 min of the applied ultrasound stimulation, and the difference was significant compared with that before the stimulation (P<0.05). The differences between the total PSD of ECoG in the other 6 time periods compared to pre-stimulation were all significant (P<0.05). Within 5 min after the cessation of ultrasound stimulation, the PSD of ECoG was 90.1±9.9% of that before stimulation, and the difference was not significant compared with that before stimulation (P>0.05).2) Ultrasound stimulation had an enhancing effect on PSD in all four frequency bands, and the difference between PSD in all four frequency bands in ultrasound stimulation and before stimulation was significant (P<0.05). After the cessation of ultrasound stimulation, there was no significant difference between the PSD of the four frequency bands compared with that before stimulation (P>0.05). 3) Mean amplitude of ECoG: Thechange of mean amplitude of ECoG in ultrasound stimulationwas significant compared with that before stimulation (P<0.05); After ultrasound stimulation was stopped, the difference was not significant compared with that before stimulation (P>0.05). 4) For the mean amplitude of the four frequency bands, ultrasound stimulation could enhance the mean amplitude of the four frequency bands. Furthermore, this enhancement was manifested in the δ band up to 10 min, and the enhancement in the θ, α and β bands was less than 5 min. Ultrasound stimulation regulated ECoG in rat prefrontal cortex and this regulation has the characteristics of promptness and rapid recovery.
王君, 随力, 吴永亮, 郑政. 超声刺激对大鼠前额叶皮层ECoG的调控[J]. 中国生物医学工程学报, 2021, 40(2): 195-201.
Wang Jun, Sui Li, Wu Yongliang, Zheng Zheng. Ultrasound Stimulation on ECoG in Rat Prefrontal Cortex. Chinese Journal of Biomedical Engineering, 2021, 40(2): 195-201.
[1] 黎国锋,邱维宝,钱明,等. 超声神经调控技术与科学仪器[J]. 生命科学仪器,2017, 1(15): 6-11. [2] 王君,随力,蔡爱楠,等. 超声刺激参数在超声神经调控中的作用[J]. 中国医学物理学杂志, 2018(2):237-242. [3] Velling VA, Shklyaruk SP. Modulation of the functional state of the brain with the aid of focused ultrasonic action[J]. Neuroscience & Behavioral Physiology, 1988, 18(5):369-375. [4] Yuan Yi, Yan Jiaqing, Ma Zhitao, et al. Noninvasive focused ultrasound stimulation can modulate phase-amplitude coupling between neuronal oscillations in the rat hippocampus[J]. Frontiers in Neuroscience, 2016, 10(191):348-354. [5] Legon W, Sato TF, Opitz A, et al. Transcranial focused ultrasound modulates the activity of primary somatosensory cortex in humans[J]. Neurosurgery, 2014, 74(6):322-329. [6] 茅正梅,李葆明. α_2肾上腺素受体与前额叶皮层认知功能[J]. 生理科学进展,1999(1):17-22. [7] Castellanos FX, Tannock R. Neuroscience of attention-deficit/ hyperactivity disorder: The search for endophenotypes.[J]. Nature Reviews Neuroscience, 2002, 3(8):617-628. [8] 刘兆瑞,张雪寒. 用光遗传方法激活前额叶皮层对脑区c-Fos表达的影响[J]. 扬州大学学报(农业与生命科学版), 2017(3):11-16. [9] 陈帅禹,庄乾,Jackson T,等. 前额叶的无创神经干预对饮食控制的影响:基于rTMS和tDCS研究的综述[J]. 心理科学, 2017(6):1511-1517. [10] 黄思佳,随力,任杰,等. 恐惧记忆的形成对大鼠内侧前额叶皮层ECoG的影响[J]. 生物医学工程学进展, 2015, 36(2):86-90. [11] 胡胜男,吴永亮,张荥娟,等. 一种用于脑神经刺激的程控超声发生器[J]. 中国生物医学工程学报, 2017, 36(5):589-595. [12] Mueller J, Legon W, Opitz A, et al. Transcranial focused ultrasound modulates intrinsic and evoked EEG dynamics[J]. Brain Stimul, 2014, 7(6): 900-908. [13] Paxinos G, Watson C. The rat brain in stereotaxic coordinates fourth edition[J]. Rat Brain in Stereotaxic Coordinates, 1998, 3(2):6-54. [14] 王勇. 基于经颅超声刺激的脑调控系统设计及实验研究[D]. 秦皇岛:燕山大学, 2014. [15] 伍少玲,燕铁斌,马超,等. 三种量表评定脑卒中急性期患者姿势控制能力的分析研究[J]. 中华物理医学与康复杂志, 2006, 28(1):39-41. [16] King RL, Brown JR, Newsome WT, et al. Effective parameters for ultrasound-induced in vivo neurostimulation[J]. Ultrasound Med Biol, 2013, 39(9): 312-331. [17] Ye PP, Brown JR, Pauly KB, et al. Frequency dependence of ultrasound neurostimulation in the mouse brain[J]. Ultrasound Med Biol, 2016, 42(7): 1512-1530. [18] Kim H, Chiu A, Lee SD, et al. Focused ultrasound-mediated non-invasive brain stimulation: examination of sonication parameters[J]. Brain Stimulation, 2014, 7(5):748-756. [19] White PJ, Clement GT, Hynynen K, et al. Local frequency dependence in transcranial ultrasound transmission[J]. Phys Med Biol, 2006, 51(9): 2293-2305. [20] Bystritsky A, Korb AS, Douglas PK, et al. A review of low-intensity focused ultrasound pulsation[J]. Brain Stimul, 2011, 4(3): 125-136. [21] Nyborg WL. Biological effects of ultrasound: Development of safety guidelines. Part II: General review[J]. Ultrasound in Medicine & Biology, 2000, 27(3):301-333. [22] Gibbs FA, Gibbs EL, Lennox WG. Effect on the electroencephalogram of certain drugs which influence nervous activity[J]. Pneumonologia Polska, 1937, 48(11): 739-742.
