Precise Optimization of 3D Bio-Printing Cell-Laden Hydrogel Construct
Huang Mengjie1 , Luo Li1, Wang Ling1,2*, Xu Mingen1,2*
1(School of Life Information Instrument and Science Engineering, Hangzhou Dianzi University, Hangzhou 310018, China) 2(Zhejiang Provincial Key Lab of Medical Information and Three-Dimensional Bio-Printing, Hangzhou 310018, China)
Abstract：The combination of 3D bio-printing technology and hydrogel provides an attractive solution for manufacturing tissues and organs with complex structures and functions. The internal constructs can be custom-printed to imitate the 3D microenvironment of tissues and organs, which is critical for cell growth, tissue formation and regeneration after printed. However, precise matching between the printed and the design is still challenging due to variable physical and chemical properties of hydrogel. In this paper, an optimized method based on optical coherence tomography (OCT) for 3D bio-printing cell-laden hydrogel construct was proposed. The tissue was imaged by 3D non-destructive homemade OCT system, and quantitatively characterized, then the mismatch of structural parameters between the design and the printed was reduced using the experimentally obtained equations, and the accuracy and stability of 3D bio-printing were improved. The results demonstrated that the deviation of the key structural parameters between the printed and the design was controlled to less than 7%, which was much lower than the deviation about 40% reached by conventional 3D bio-printing. The controlled parameters included pore size, strut size, porosity, surface area and pore volume. And the cell viability of two weeks incubation was increased from about 80% to over 90%. It was concluded that online quantitative evaluation and feedback iterative analysis system based on OCT provided a potential tool for mass customization of cell-laden hydrogel tissues, 3D bio-printing tissues and organs.
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