Study on 3D Bioprinting of Liver Tissues Based on Coaxial Flow Technique
Du Xianbin1, Xu Mingen1,2*, Wang Ling1,2, Zhou Yongyong3
1School of Life Information Instrument and Science Engineering, Hangzhou Dianzi University, Hangzhou 310018, China; 2Zhejiang Provincial Key Lab of Medical Information and Three-Dimensional Bio-Printing, Hangzhou 310018, China; 3Hangzhou Regenovo Biotechnology Co., Ltd., Hangzhou 310018, China
Abstract：The 3D bioprinting provides a new technical possibility for the medical field, which can be widely used in the manufacture of artificial tissues and organs. However, the function and size of the artificial tissue are limited by the vascularization of the tissue. In this study, a coaxial flow extrusion system was used to fabricate hollow filaments in encapsulated liver cells, combined with a biological 3D printing system, so as to fabricate liver tissue with microchannel network by using a layer-by-layer approach. In the experiment, an integrated coaxial flow 3D bioprinting system was built firstly. The influence of material extrusion rate and material concentration was then studied on the size of hollow filaments and the velocity of wire. Subsequently, the biomimetic liver tissue containing multi-layer networks was printed with the liver cell line C3A as the material. Finally, the biomimetic liver tissue containing microchannels was divided into groups and cultured. The cell survival rate of hepatocytes after 24h, 48h and 72h of culture was detected by live-dead cell staining in perfusion group and non-perfusion group. The experimental results showed that in the organs using 3D printing technology based on coaxial flow technique, the hollow filaments could be effectively fused, and the 3D microchannel network inside the support was complete. Besides, the proportion of damaged hepatocyte was less in the printing process, and the survival rate of hepatocytes was over 90% in hollow filaments. Furthermore, there was a significant difference in the cell survival rate between the perfusion group and the non-perfusion group after culture for 72h, suggesting that microchannel perfusion could promote the exchange of material within the tissue and increase the survival rate of liver cells around the microchannel. The printing technology and perfusion system presented in this paper provide a new study approach for the vascularization and culture of artificial tissues.
杜显彬, 徐铭恩, 王玲, 周永勇. 基于同轴流技术的肝组织生物3D打印研究[J]. 中国生物医学工程学报, 2018, 37(6): 731-738.
Du Xianbin, Xu Mingen, Wang Ling, Zhou Yongyong. Study on 3D Bioprinting of Liver Tissues Based on Coaxial Flow Technique. Chinese Journal of Biomedical Engineering, 2018, 37(6): 731-738.
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