Abstract：In this work, characteristics of local field potential before and after fear memory formation were studied to investigate the effects of fear memory formation on local field potential in rat medial prefrontal cortex. Eight healthy adult male SD rats were selected. The electrodes were implanted into the medial prefrontal cortex of the rats by stereotaxic apparatus. After one week, shuttle box test was used to induce fear memory in rats when rats were awake. The local field potential of rat medial prefrontal cortex was monitored by using Cerebus neural signals recording system, before and 6 h, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d after fear memory formation. The Neuroexplorer software was introduced to analysis the local field potential. Power spectral density and power percentage of local field potentials were analyzed and compared with total power before and after the formation of fear memory and among different frequency bands (δ: 1-3 Hz, θ: 4-7 Hz, α: 8-13 Hz, β: 14-30 Hz, γ: 31-100 Hz). The results showed that the power percentage of θ and β at 6 h after fear memory formation was significantly different from that of before fear memory formation (P=0.017, P=0.017); The power percentage of θ was increased than that before fear memory formation. The power percentage of β was reduced than before fear memory formation. In addition, the power percentage of δ 1-2 days after fear memory formation was significantly different from that before fear memory formation (P=0.03, P=0.049). The power percentage of γ on 1～2 days after fear memory formation was significantly different from that before fear memory formation (P=0.013, P=0.019).These results indicated that activating mode of neural information processing in rat medial prefrontal cortex was significantly altered following fear memory formation.
张丙淑, 随 力, 黄思佳. 恐惧记忆的形成对大鼠内侧前额叶皮层局部场电位的影响[J]. 中国生物医学工程学报, 2017, 36(1): 53-58.
Zhang Bingshu, Sui Li, Huang Sijia. Effects of Fear Memory Formation on Local Field Potential in Rat Prefrontal Cortex. Chinese Journal of Biomedical Engineering, 2017, 36(1): 53-58.
[1] Scherberger HJ, Jarvis MR, Andersen RA. Cortical local potential encodes movement intentions in the posterior parietal cortex [J]. Neuron,2005, 46(2):347-354. [2] 刘国庆.大鼠视觉感受区电位信号与视觉刺激之间关系研究方案综述 [J].数学的实践与认识,2015, 45(14):132-135. [3] Logothetis NK, Kayser C, Oeltermann A. In vivo measurement of cortical impedance spectrum in monkeys [J]. Neuron, 2007, 55(5):684-686. [4] Bai Wenwen, Liu Tiaotiao, Yi Hu, et al. Anticipatory activity in rat medial prefrontal cortex during a working memory task [J]. Neuroscience Bulletin, 2012, 28(6):693-703. [5] David R, Sean MM, Alexander T, et al. Cannabinoids reveal importance of spike timing coordination in hippocampal function [J]. Nat Neurosci, 2006, 9(12):1526-1533. [6] Siegel M, Warden MR, Miller EK. Phase-dependent neuronal coding of objects in short-term memory [J].Proc Natl Acad Sci USA,2009,106(50):21341-21346. [7] Hasselmo ME, Bodelon C, Wyble BP. A proposed function for hippocampal theta rhythm: separate phases of encoding and retrieval enhance reversal of prior learning [J].Neural Comput,2002, 14(4):793-817. [8] Benchenane K, Peyrache A, Khamassi M, et al. Coherent thete oscillations and reorganization of spike timing in the hippocampal-prefrontal network upon learning [J]. Neuron, 2010, 66(6):921-936. [9] 张方凤,郑志刚.复杂脑网络研究:现状与挑战 [J].上海理工大学学报,2012,34(2):138-153. [10] Wellman LL, Mairen EF, Mayumi M, et al. The basolateral amygdala determines the effects of fear memory on sleep in an animal model of PTSD [J]. Experimental Brain Research,2014, 232(5):2255-2265. [11] David NF, Tanya SH, Ashleigh RT, et al. An IDEA for short term out break projection: nearcasting using the basic reproduction number [J]. PLoS ONE, 2013, 8(12): 1-8. [12] 刘文义.条件化恐惧消退的神经机制研究进展 [J].滨州学院学报,2008,24(3):62-65. [13] 杨东升,刘晓莉,乔德才,等.性疲劳形成和恢复过程ECoG的动态研究 [J].体育科学,2012,32(4):53-59. [14] Breakspear M, Williams LM, Stam CJ. A novel method for the topographic analysis of neural activity reveals formation and dissolution of ‘dynamic cell assemblies’ [J]. Compute Neuroscience,2004,16(1):49-68. [15] Muthuswamy J, Thakor NV. Spectral analysis methods for neurological signals [J]. Neuroscience Methods,1998,83(1):1-14. [16] Makeig S, Inlow M. Lapses in altertness: coherence of fluctuations in performance and EEG spectrum [J]. Electroencephalography and Clinical Neurophysiology,1993, 86(1):23-35. [17] 孔莉,戴体俊,郭忠民.伤害性刺激对家兔定量药物脑电δ频段的影响[J].国际麻醉学与复苏杂志,2009,30(6):515-517. [18] 丁锦红,郭春彦.工作记忆的脑机制研究[J].心理科学,2001,24(5):583-585.
