Abstract：To explicitly understand the internal changes of gastrointestinal tissue compressed by stapler is a great precondition for optimizing the stapler’s engineering parameters. However, relevant work has not been adequate. By a modified stapler with embedded detection electrodes, the multi-frequency bio-impedance of porcine small intestine under compression of the stapler was measured. The ColeYmodel was used to fit the measurement results and changes within the tissue were analyzed according parameters of the model. G0 represents the extracellular fluid conductance and ΔG was the intracellular fluid conductance. The changes of G0 and ΔG under different compression strength were acquired by using spacers with different thickness. It turned out that the whole squeezing process was divided into two stages. At the first stage, two parameters were both falling fast. At the second stage, with the increase of compression strength, G0 kept decreasing and the trend of ΔG was changed from a slight fluctuation into a slight decline. When the thickness of spacer increased from 0 mm to 0.52 mm, the percentage of samples with highly significant correlation between pressure and ΔG in all samples was increased from 40% to 100%, however, the percentage for G0 was constant as 100%. It was concluded that under the effect of stapler, a part of tissue of the small intestine was firstly squeezed out of the measurement space, which led to a rapid decline of parameters. After that, with stapler continuous pressing the tissue, extracellular fluid was kept discharged, and intracellular fluid showed different trends under different pressing strength, which might be related to the compression deformation of cells. This research provided a basis for optimizing the parameters of a stapler.
周 宇, 张 宽, 许晶晶, 任彬彬, 李博婷, 宋成利. 吻合器作用下小肠组织细胞内外液变化研究[J]. 中国生物医学工程学报, 2017, 36(1): 59-66.
Zhou Yu, Zhang Kuan, Xu Jingjing, Ren Binbin, Li Boting, Song Chengli. Research on the Changes of Intra-and Extra-Cellular Fluid within Small Intestine under Compression of Stapler. Chinese Journal of Biomedical Engineering, 2017, 36(1): 59-66.
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