重力矢量及趋化诱导对脂肪干细胞在PLGA电纺膜上早期增殖的影响

doi:10.3969/j.issn.0258-8021. 2014. 04.018

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摘要观察研究重力矢量方向及血小板衍生生长因子（PDGF）趋化诱导，在体外环境对人脂肪干细胞（ASCs）在PLGA电纺膜表面黏附和增殖活动的影响。体外原代培养从人颊脂垫提取的ASCs，根据不同重力矢量条件以及有无趋化因子PDGF将实验分为4个组。A组：PDGF/重力矢量（+），含PDGF的PLGA电纺膜置于ASCs悬液底部；B组：PBS/重力矢量（+），空白（PBS）电纺膜置于ASCs悬液底部；C组：PDGF/重力矢量（-），含PDGF的PLGA电纺膜覆于ASCs悬液表面；D组：PBS/重力矢量（-），空白（PBS）电纺膜覆于ASCs悬液表面。分别于0、1、3、5、8、11 d加入Alamar Blue继续孵化24 h后测OD值，对照标准曲线，计算各组Alamar Blue的下降百分比，评估ASCs的黏附增殖活动。结果显示：在重力矢量（+）组的早期（第1～4 d），无论趋化因子PDGF存在与否，Alamar Blue的下降百分比都能达到30%左右，不影响细胞的定植、黏附与增殖。但是在重力矢量（-）组，缺少趋化因子PDGF的Alamar Blue的下降百分比为25%左右，细胞将无法正常的定植与黏附。PDGF具有明显诱导ASCs趋化聚集作用，能减轻不利重力因素的影响，显著改善ASCs黏附与增殖。

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	钟晓敏陈刚*张圣敏乔峰

关键词 ：脂肪干细胞, 血小板衍生生长因子, 重力矢量, 黏附, 增殖

Key words： adiposederived stem cells (ASCs) plateletderived growth factor (PDGF) gravity vector adhesion proliferation

基金资助:教育部留学回国人员科研启动基金资助项目（教外司留(2012)940）

引用本文:

钟晓敏陈刚*张圣敏乔峰. 重力矢量及趋化诱导对脂肪干细胞在PLGA电纺膜上早期增殖的影响[J]. 中国生物医学工程学报, 2014, 33(4): 508-512.
ZHONG Xiao Min CHEN Gang*ZHANG Sheng Min QIAO Feng. Influences of Gravity Vector and Chemotaxis on ASCs Proliferation onto PLGA Electrospinning Membrane in Early Stage. journal1, 2014, 33(4): 508-512.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021. 2014. 04.018 或 http://cjbme.csbme.org/CN/Y2014/V33/I4/508

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