纳米羟基磷灰石/壳聚糖( n-HA/CS) 复合材料对SD 大鼠代谢器官的影响

doi:10.3969/j.issn.0258-8021.2013.05.012

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摘要纳米羟基磷灰石/壳聚糖复合材料（n-HA/CS）是目前骨修复替代领域使用广泛的一种生物材料。为探讨该材料在体内经代谢降解后，对大鼠体内代谢器官生理功能的影响，并分析其可能的作用机制。本研究用共沉淀法制备nHA/CS复合材料，并采用红外光谱分析（IR）、X射线衍射（XRD）等手段表征该材料。通过细胞生长抑制实验，病理切片以及血清生化指标的检测，从体内体外两种方式观察该材料对MC3T3-E1细胞以及SD大鼠肝脏、肾脏等代谢器官的影响。结果显示，100 mg/kg含量nHA/CS复合材料粉末在大鼠体内代谢8周，血清尿素氮（BUN）水平升高为29.6 mmol/L；肌酐（CR）水平升高为66.9 umol/L；总胆红素（T-B）及间接胆红素（I-B）水平也分别升高至1.79 umol/L和1.32 umol/L，均较对照组水平明显增高，有显著性差异(P≤0.05)。且肝脏、肾脏组织中出现大量凋亡细胞，无组织坏死和炎症表现。n-HA/CS复合材料在体内代谢8周后，通过诱导细胞凋亡影响机体肝、肾等代谢器官的功能。

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	王丽婷¹
	2周钢¹樊瑜波^1*

关键词 ：纳米羟基磷灰石/壳聚糖复合材料, 凋亡, 代谢

Abstract：Composite conmposed of nanohydroxyapatite and chitosan (n-HA/CS) is widely used in the field of bone repair and alternative. To investigate the degradation effects of nHA/CS on metabolism organs in SD rat and analyze the possible mechanism, particulate n-HA/CS was prepared by precipitation and characterized by IR and XRD. Serum biochemical parameters measurement and histopathological analysis were performed to evaluate the composite toxicity after 8 weeks of composite implantation (100 mg/kg). Results show that blood urea nitrogen (BUN) levels was 29.6 mmol/L, creatinine (CR) levels was 66.9 umol/L, total bilirubin (T-B) and indirect bilirubin (I-B) levels were also increased to 1.79 umol/L and 1.32umol/L. Compared with that of control, there were significant differences (P≤0.05). A large number of apoptotic instead of necrosis cells were observed in the liver and renal proximal tubule tissue. It is concluded that the toxicity of n-HA/CS is attributable to the cells apoptosis.

Key words： nano-hydroxyapatite/chitosan composite apoptosis metabolism

基金资助:国家自然科学基金重大国际合作研究项目（11120101001）；国家自然科学基金（10925208）

引用本文:

王丽婷^1，2周钢¹樊瑜波^1*. 纳米羟基磷灰石/壳聚糖( n-HA/CS) 复合材料对SD 大鼠代谢器官的影响[J]. 中国生物医学工程学报, 2013, 32(5): 595-600.
WANG Li Ting^1，2 ZHOU Gang¹FAN Yu Bo^1*. Effects of Nano-Hydroxyapatite/Chitosan (N-HA/CS) on MC3T3-E1 Cell and Metabolic Organ in SD Rats. journal1, 2013, 32(5): 595-600.

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http://cjbme.csbme.org/CN/ 10.3969/j.issn.0258-8021.2013.05.012 或 http://cjbme.csbme.org/CN/Y2013/V32/I5/595

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