Abstract：The aim of this work is to explain the mechanisms of electrophysiological changes in rat blood cell by using RC equivalent circuit model under simulated weightlessness. Simulating weightlessness model was established by rat tail suspension. Electrical impedance spectroscopy of rat blood on the control and tail suspension group was performed using Aligent 4294A impedance analyzer. Electrical parameters (resistivity and capacitance) of blood were compared between the control and tail suspension through numerical calculation of equivalent circuit model and curve fitting analysis. After 60 days post simulating weightlessness, decrease in the hematocrit (Hct) and resistivity of the erythrocyte membrane were compared with that of normal group. Experimental reuslts showed that the decrease in extracellular and intracellular resistivity was 16.44% and 1.54% respectively, the cell membrane and intracellular capacitance decreased by 4.66% and 0.83% respectively, but extracellular capacitance remained unchanged. The rat blood hematocrit (Hct) and erythrocyte membrane resistivity were significantly reduced in the simulating weightlessness model, which led to reduction in electrical impedance of the extracellular fluid and whole blood, thus gave rise to electrical conductivity. The electrical impedance spectroscopy moved to low resistance direction.
宫宇 沈斌 陈林 方云 马青*. 模拟失重对大鼠血液电阻抗谱影响的实验研究[J]. 中国生物医学工程学报, 2012, 31(6): 853-858.
GONG Yu SHEN Bin CHEN Lin FANG Yun MA Qing*. The Experimental Study of Effect of Simulated Weightlessness on Electrical Impedance Spectroscopy of Rat Blood. journal1, 2012, 31(6): 853-858.
［1］冯岱雅，孙喜庆，卢虹冰. 失重对人体循环系统影响的仿真研究［J］. 第四军医大学学报，2005，26(16)：1526-1528.
［2］Balakhovshii IS, Noskov VB. Hemoglobin mass in humans during a space flight and its simulation［J］. Aviakosm Ekolog Med, 2008, 42(6): 32-44.
［3］孟京瑞，沈羡云，董颀，等. 7天模拟失重对人体血液流变性的影响［J］. 中国微循环，1997，1(2)：77-79.
［4］陈杰，马进，丁兆平，等. 一种模拟长期失重影响的大鼠尾部悬吊模型［J］. 空间科学学报，1993，13(2)：159-162.
［5］郭芮，胡敏，孙振宇，等. 模拟失重对大鼠下颌骨、腰椎和股骨组织结构的影响 ［J］. 航天医学与医学工程，2005，18(3)：165-169.
［6］周党侠，邱曙东，王志勇，等. 尾部悬吊状态对性成熟期雄性大鼠生殖功能的影响［J］. 中华男科学杂志，2006，12(4)：326-329.
［7］崔彦，董家鸿，周金莲，等. 模拟失重对大鼠Hsp及其基因表达的影响［J］. 中华肝胆外科杂志，2010，3(1)：48-54.
［8］MJ Xie, YG Ma, F Gao, et al. Activation of BKCa channel is associated with increased apoptosis of cerebrovascular smooth muscle cells in simulated microgravity rats［J］. Am J Physiol Cell Physiol, 2010, 298(6): C1489-C1500.
［9］Nicogossian AE, Huptoon CL, Pool SL. Space physiology and medicine［M］. Philadelphia: Lea & Febiger, 1994: 334-350.
［10］Scribner Associates Inc. ZPlot for Windows Software ［EB/OL］. http://www.scribner.com/zviewforwindows.html, 2012-06-01
［11］Foster KR, Schwan HP. Dielectric properties of tissues［M］//Polk C, Postow E, ed. Handbook of Biological Effects of Electromagnetic Fields. (2nd Edition). Boca Raton: CRC Press, 1996: 25-104.
［12］彭远开，张静雪，王承珉，等. 模拟失重对大鼠血清丙二醛含量的影响［J］. 航天医学与医学工程，1996，9(1)：54-56.
［13］郭凯，胡华碧，陈琳，等. 二十二碳六烯酸和二十碳五烯酸对模拟失重大鼠红细胞膜流动性的影响［J］. 细胞与分子免疫学杂志，2009，25(9)：844-846.
［14］Ivanova SM, Maksimov GV, Morukov BV, et al. Role of viscosity and permeability of erythrocyte plasmatic membrane in controlling the oxygen transport effectiveness by human hemoglobin on completion of space flight［J］.Aviakosm Ekolog Med, 2007, 41(2): 41-44.
［15］De Santo NG, Cirillo M, Kirsch KA, et al. Anemia and erythropoietin in space flights［J］. Seminars in Nephrology, 2005, 25(6): 379-387.
［16］Rice L, Ruiz W, Driscoll T. Neocytolysis on descent from altitude: a newly recognized mechanism for the control of red cell mass［J］. Ann Intern Med, 2001, 134(8): 652-656.［17］陈建和，沈羡云，孟京瑞，等. 模拟失重对兔红细胞形态及生成的影响［J］．航天医学与医学工程，1995，8(4)：283-285.
［18］Schwan HP, Electrical properties of blood and its constituents［J］. Blut, 1983,〖STHZ〗46〖STBZ〗(4): 185-197.
［19］陈林，马青，王力，等. 大鼠红细胞悬浮液阻抗谱 ColeCole数学模型分析［J］. 航天医学与医学工程. 2010，23(3)：182-187.
［20］马青，洲崎敏伸. 家兔红细胞阻抗谱的实验和等效电路模型拟合研究［J］. 中国生物医学工程学报，2006，25（5）：566-570
［21］王力，陈林，王琳，等. 小鼠血液阻抗频谱特性及其等效电路模型分析［J］. 宁波大学学报(理工版)，2009，22(4)：573-576.
