基于光电倍增管的化学发光免疫检测系统的研制

doi:10.3969/j.issn.0258-8021.2012.06.009

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摘要基于光电倍增管(PMT)设计了全自动化学发光免疫检测总体方案，以泌乳素(PRL)作为检测对象，标记酶采用辣根过氧化物酶(HRP），发光体为Pierce的鲁米诺（激发底物），对化学发光免疫检测系统PMT数据进行处理。通过检测5种不同浓度的HRP稀释液的发光数进行标定，后对数据进行线性化处理，得到PRL浓度与发光数的关系。两检测样本PRL分别取阳性范围浓度值（样本A）和阴性范围浓度值（样本B），同时将检测结果与 LUMO与 Liaison两种化学发光仪的检测结果进行比较。对于样本A，本系统、LUMO和Liaison的检测结果分别为51.724、 46.198、43.792 ng/mL；对于样本B，本系统、LUMO和Liaison的检测结果分别为11.702、15.133、 8.069 ng/mL。实验结果表明，所设计的全自动化学发光免疫检测系统可以确定被测样本的阴阳性，能够满足免疫分析应用需求。

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	任玉强¹李丽孙福佳¹
	2
	3*陈烁烁¹蔡锦达¹
	3尤黔林¹

关键词 ：化学发光免疫分析仪, 光电倍增管(PMT), 信号检测系统

Abstract：In this work a full automatic chemiluminescence immune analyzer was designed and fabricated base on photomultiplier tube (PMT). Experiments were conducted in which the prolactin (PRL) was taken as a detection object, the Horse Radish Peroxidase (HRP) served as Markey enzyme, and the Pierce Luminol (excitation substrate) was employed as a light resource. The PMT data obtained by the chemiluminescence immune detection system was processed, calibrated through the number of light emitting detection of five different consistency of PRL.The relationship between the consistency of PRL and the lightemitting was obtained. Samples in positive range of consistency (sample A) and negative range of consistency (sample B) and samples for LUMO and Liaison were measured. The results of sample A obtained from the present system, LUMO and Liaison were 51.724, 46.198, 43.792 ng/mL respectively, the data of sample B were 11.702, 15.133, 8.069 ng/mL respectively. It showed that the automatic chemiluminescence immune system can measure the negative or positive of samples and meet the application requirements of the immunoassay through the comparison with other chemiluminescence analyzers.

Key words： chemiluminescence immunoassay analyzer (CLIA) photomultiplier tube (PMT) signal acquisition system

基金资助:国家重大科学仪器设备开发专项(2011YQ150040)；2011年度上海市重大技术装备研制专项(ZB-ZBYZ-03-11-1709)

引用本文:

任玉强¹李丽孙福佳^1,2,3*陈烁烁¹蔡锦达^1,3尤黔林¹. 基于光电倍增管的化学发光免疫检测系统的研制[J]. 中国生物医学工程学报, 2012, 31(6): 859-865.
SUN Fu Jia^1,2,3*CHEN Shuo Shuo¹CAI Jin Da^1,3YOU Qian Lin¹. Development of Chemiluminescence Immune Detection System Based on Photomultiplier Tube. journal1, 2012, 31(6): 859-865.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2012.06.009 或 http://cjbme.csbme.org/CN/Y2012/V31/I6/859

［1］Luo Gangyin, Wang Bidou, Yuan Chunhui, et al. The key technologies of a new semiautomatic chemiluminescence immunoassay analyzer based on the photomultiplier tube［J］. IEEE Advanced Computer Theory and Engineering, 2010, 6:402-405.
［2］Kawabata R, Mizoguchi T, Tsukamoto A, et al. Immunoassay detection without washing by using AC magnetic susceptibility［J］. IEEE Sensors, 2010:727-731.
［3］Yin CH, Tisa YY, Cho HJ, et al. Microfluidic device by using nanomagnetic beads conjugated antibodies for immunoassay［J］. IEEE International Conference on Nano/Molecular Medicine and Engineering, 2009:57-60.
［4］Zeng Nianyin, Wang Zidong, Li Yurong, et al. Identification of nonlinear lateral flow immunoassay statespace models via particle filter approach［J］. IEEE Transactions on Nanotechnology, 2012, 11:321-327.
［5］Kiely J, Hawkins P, Wraith P, et al. Paramagnetic particle detection for use with an immunoassay based biosensor［J］. IEEE Science Measurement and Technology, 2007, 1:270-275.
［6］张建鹏. 全自动化学发光免疫分析仪［D］. 天津: 天津大学, 2005.
［7］郭长江. 全自动生化分析仪的研制［D］. 武汉: 华中科技大学, 2005.
［8］Rowe CA, Scruggs SB, Feldstein MJ, et al. An array immunosensor for simultaneous detection of clinical analysis［J］. Anal Chem, 1999, 71:305-308
［9］Moignea FI, Beauvieux MC, Derache P, et al. Determination of myoglobin comparative evaluation of the new automated VIDAS assay with two other immunoassays［J］. Clin Biochem，2002, 35:255-262
［10］Yao Yufeng, Lu Shizhou, Huang Bo, et al. Automated chemiluminescence immunoassay analyzer［J］. IEEE Intelligent Computation Technology and Automation, 2010, 1:829-832.
［11］Popov V, Majewski S, Wojtsekhowski B, et al. A simple method to increase effective PMT gain by amplifier circuit oowered from voltage divider［J］. IEEE Nuclear Science Symposium Conference Record, 2002, 2:634-637.
［12］Yang Yongfeng, Wu Yibao, Cherry R. Investigation of depth of interaction encoding for a pixelated LSO array with a single multichannel PMT［J］. IEEE Transactions on Nuclear Science, 2009, 56:2594-2599.
［13］Siegel S, Silverman RW, Shao Yiping, et al. Simple charge division readouts for imaging scintillator arrays using a multichannel PMT［J］. IEEE Transactions on Nuclear Science and Medical Imaging Conference Record, 1996, 1:13-17.
［14］Yao ChunJiang, Wang Xuping, Chen Xiaohu, et al. Design of embedded signal acquisition system for fiber optic sensor［J］. IEEE Intelligent Information Technology Application, 2009, 1:121-125.
［15］高荣, 赵一泽. 化学发光免疫分析技术应用研究新进展［J］. 中国生物制品学杂志, 2008, 21(4):338-342.
［16］Yang Yingchun, Feng Yuanyuan, Ye Zhixiang, et al. Development of a new flowinjection chemiluminescence method for the determination of sulfide［J］. IEEE Bioinformatics and Biomedical Engineering, 2010:1-4.
［17］Zhu Jiwei. The development and design of the chemiluminescence detection system based on DSP［J］. IEEE Computer, Mechatronics, Control and Electronic Engineering, 2010, 4:214-216.
［18］Xu Wei, Wei Yanchun, Xing Da, et al. Quantification of human serum albumin by highly sensitive chemiluminescence method［J］. IEEE Complex Medical Engineering, 2007:1694-1697.