|
|
|
| Advances in the Study of Liver-on-Chips with Bionic Microfluidic |
| Li Haidi, Chen Xiaoping* |
| (College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310000,China) |
|
|
|
|
Abstract One of the recent frontier hotspots in biomedical field is organ-on-chips with bionic microfluidic. The chips comprise of micro-tissues that mimic in vivo organs and are able to realize high-throughput detection, which have potential wide applications in the drug discovery and toxicity testing. The liver, a metabolic center of human body, is sensitive for chemicals poison and essential to evaluate the hepatotoxicity in toxicity testing. In recent years the advance in this field come out a great deal of results in liver-on-chips with bionic microfluidic. In this review, we provided an overview of the design concepts, micro-channel perfusion methods and cell culture systems. We also discussed the application prospects and the future trends of liver-on-chips.
|
|
Received: 02 February 2012
|
|
|
|
|
|
[1] Usta OB, Mccarty WJ, Bale S, et al. Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution of in-vitro liver technologies[J]. Technology, 2015, 3(1):1-26.
[2] 孙威, 陈雨晴, 罗国安,等. 器官芯片及其应用[J]. 分析化学, 2016, 44(4):533-541.
[3] Zhang J, Zhao X, Liang L, et al. A decade of progress in liver regenerative medicine.[J]. Biomaterials, 2018, 157:161-176.
[4] An F, Qu Y, Liu X, et al. Organ-on-a-c: New platform for biological analysis[J]. Analytical Chemistry Insights, 2015, 10(1):39-45.
[5] Chao M, Lei Z, Zhou EM, et al. On-chip construction of liver lobule-like microtissue and its application for adverse drug reaction assay[J]. Analytical Chemistry, 2016, 88(3):1719-1727.
[6] Yum K, Hong SG, Lee LP. Physiologically relevant organs on chips[J]. Biotechnology Journal, 2014, 9(1):16-27.
[7] Riahi R, Shaegh SA, Ghaderi M, et al. Automated microfluidic platform of bead-based electrochemical immunosensor integrated with bioreactor for continual monitoring of cell secreted biomarkers[J]. Scientific Reports, 2016, 6:24598.
[8] Kim D, Wu X, Young AT, et al. Microfluidics-Based in vivo mimetic systems for the study of cellular biology.[J]. Accounts of Chemical Research, 2014, 47(4):1165-1673.
[9] He J, Mao M, Liu Y, et al. Fabrication of nature-inspired microfluidic network for perfusable tissue constructs[J]. Advanced Healthcare Materials, 2013, 2(8):1108-1113.
[10] Zhou Q, Patel D, Kwa T, et al. Liver injury-on-a-chip: microfluidic co-cultures with integrated biosensors for monitoring liver cell signaling during injury[J]. Lab on a Chip, 2015, 15(23):4467-4478.
[11] Lee PJ, Hung PJ, Lee LP. An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture[J]. Biotechnology and Bioengineering, 2007, 97(5):1340-1346.
[12] Messner S, Agarkova I, Moritz W, et al. Multi-cell type human liver microtissues for hepatotoxicity testing[J]. Archives of Toxicology, 2013, 87(1):209-213.
[13] Esch MB, Prot JM, Wang YI, et al. Multi-cellular 3D human primary liver cell cultures elevate metabolic activity under fluidic flow[J]. Lab on a Chip, 2015, 15(10):2269-2277.
[14] Ljy O, Chong LH, Jin L, et al. A pump-free microfluidic 3D perfusion platform for the efficient differentiation of human hepatocyte-like cells.[J]. Biotechnology and Bioengineering, 2017, 114(10):2360-2370.
[15] Vella SJ, Beattie P, Cademartiri R, et al. Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick[J]. Analytical Chemistry, 2012, 84(6):2883-2891.
[16] Pollock NR, Colby D, Rolland JP. A point-of-care paper-based fingerstick transaminase test: toward low-cost "lab-on-a-chip" technology for the developing world[J]. Clin Gastroenterol Hepatol, 2013, 11(5):478-482.
[17] Bhise NS, Manoharan V, Massa S, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids.[J]. Biofabrication, 2016, 8(1):014101.
[18] Tong Wenhao, Fang Yu, Yan Jie, et al. Constrained spheroids for prolonged hepatocyte culture.[J]. Biomaterials, 2016, 80(2016):106-120.
[19] Liu Yaowen, Wei Jiaojun, Lu Jinfu, et al. Micropatterned coculture of hepatocytes on electrospun fibers as a potential in vitro, model for predictive drug metabolism[J]. Materials Science and Engineering C, 2016, 63(2016):475-484.
[20] Yip D, Cho CH. A multicellular 3D heterospheroid model of liver tumor and stromal cells in collagen gel for anti-cancer drug testing.[J]. Biochemical and Biophysical Research Communications, 2013, 433(3):327-332.
[21] Ma Xuanyi, Qu Xin, Zhu Wei, et al. Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(8):2206-2211.
[22] Yang Xingyuan, Xu Xinyue, Zhang Yuan, et al. 3D microstructure inhibits mesenchymal stem cells homing to the site of liver cancer cells on a microchip.[J]. Genes, 2017, 8(9):218-227.
[23] Grix T, Ruppelt A, Thomas A, et al. Bioprinting perfusion-enabled liver equivalents for advanced organ-on-a-chip applications.[J]. Genes, 2018, 9(4):176-190.
[24] Maschmeyer I, Lorenz AK, Schimek K, et al. A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents[J]. Lab on a Chip, 2015, 15(12):2688-2699.
[25] Oleaga C, Bernabini C, Smith AST, et al. Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs[J]. Scientific Reports, 2016, 6(7):20030.
[26] Li Zhongyu, Guo Yaqiong, Yu Yue, et al. Assessment of metabolism-dependent drug efficacy and toxicity on a multilayer organs-on-a-chip[J]. Integrative Biology Quantitative Biosciences from Nano to Macro, 2016, 8(10):1022-1029.
[27] Satoh T, Sugiura S, Shin K, et al. A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform[J]. Lab on a Chip, 2018, 18(1):115-125.
[28] Prodanov L, Jindal R, Bale SS, et al. Long term maintenance of a microfluidic 3-D human liver sinusoid.[J]. Biotechnology and Bioengineering, 2016, 113(1):241-246.
[29] Mi S, Yi X, Du Z, et al. Construction of a liver sinusoid based on the laminar flow on chip and self-assembly of endothelial cells[J]. Biofabrication, 2018, 10(2):025010.
[30] Choe A, Ha SK, Choi I, et al. Microfluidic Gut-liver chip for reproducing the first pass metabolism.[J]. Biomedical Microdevices, 2017, 19(1):4-14.
[31] Esch MB, Mahler GJ, Stokol T, et al. Body-on-a-Chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury[J]. Lab on A Chip, 2014, 14(16):3081-3092.
[32] Choucha SL, Aninat C, Grsicom L, et al. Investigation of ifosfamide nephrotoxicity induced in a liver-kidney co-culture biochip[J]. Biotechnology and Bioengineering, 2013, 110(2):597-608.
[33] Materne EM, Ramme AP, Terrasso AP, et al. A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing[J]. Journal of Biotechnology, 2015, 205:36-46.
[34] Bauer S, Huldt CW, Kanebratt KP, et al. Functional coupling of human pancreatic islets and liver spheroids on-a-chip: Towards a novel human ex vivo type 2 diabetes model[J]. Scientific Reports, 2018, 8(1):1672-1682.
[35] Vernetti LA, Senutovitch N, Boltz R, et al. A human liver microphysiology platform for investigating physiology, drug safety, and disease models[J]. Experimental Biology and Medicine, 2016, 241(1):101-114.
[36] Skardal A, Devarasetty M, Soker S, et al. In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device[J]. Biofabrication, 2015, 7(3):031001.
[37] Jie Mingsha, Mao Sifeng, Liu Hanyang, et al. Evaluation of drug combination for glioblastoma based on an intestine-liver metabolic model on microchip.[J]. Analyst, 2017, 142(19):3629-3638.
[38] Li Zhongyu, Lei Jiang, Zhu Yujuan, et al. Assessment of hepatic metabolism-dependent nephrotoxicity on an organs-on-a-chip microdevice.[J]. Toxicol in Vitro, 2018, 46(2018):1-8.
[39] Shin SR, Kilic T, Zhang YS, et al. Label-free and regenerative electrochemical microfluidic biosensors for continual monitoring of cell secretomes[J]. Advanced Science. 2017, 4(5):1600522.
[40] Bavli D, Prill S, Ezra E, et al. Real-time monitoring of metabolic function in liver-on-chip microdevices tracks the dynamics of mitochondrial dysfunction.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(16):2231-2240.
[41] Rennert K, Steinborn S, Gr ger M, et al. A microfluidically perfused three dimensional human liver model.[J]. Biomaterials, 2015, 71(2015):119-131.
[42] Lin C, Khetani SR. Advances in engineered liver models for investigating drug-induced liver injury[J]. Bio Med Research International, 2016, 2016(14):1-20.
[43] Bale SS, Vernetti L, Senutovitch N, et al. In vitro platforms for evaluating liver toxicity.[J]. Experimental Biology and Medicine, 2014, 239(9):1180-1191.
[44] Gr ger M, Dinger J, Kiehntopf M, et al. Preservation of cell structure, metabolism, and biotransformation activity of liver-on-chip organ models by hypothermic storage.[J]. Advanced Healthcare Materials, 2017,242(10):1593-1604.
[45] Yu Fang, Deng Rensheng, Tong Wenhao, et al. A perfusion incubator liver chip for 3D cell culture with application on chronic hepatotoxicity testing:[J]. Scientific Reports, 2017, 7(1):14528. |
|
|
|
