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| An Experimental Study on Optimal Ratio of Chondrogenesis in Pellet Co-Culture of Adipose-Derived Stem Cells and Chondrocytes Differentiation in Rabbits |
| Zhao Mingcan1 Yang Wu1 Liu Chang1,2* |
1School of Stomatology, Jilin University, Changchun 130021, China
2Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China |
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Abstract The aim of this work was to figure out the optimal ratio of adipose-derived stem cells (ADSCs) to articular chondrocyte (AC) (ADSCs/AC)in the chondrogenesis effect through indirectly co-culture pellets of ADSCs and AC. Isolate and cultivate ADSC and chondrocytes to form pellets that were placed into upper and lower layers of tranwell system. Experiments included positive control group, negative control group, growth factor control groupand ADSC and chondrocytes pellets in different ratios (0.5∶1; 1∶1; 1∶2; 1∶3; 1∶5; 1∶7) groups. After 28 days of culture,histological morphology, protein level of GAG, COL-II and COL-X were examined. Results: Toluidine blue staining and collagen typeII displayed 0.5∶1 and 1∶1 groups showed weak positive staining. The collagen X in the growth factor group showed the most strong positive staining; DMMB showed that the total GAG content in co-culture groups increased when compared with that of the control group (P<0.05),while decreased whenthe ratio of ADSC/AC reached 1∶5. Results obtained from OHP measurements howed the variation tendency of total collagen was similar to the change tendency of GAG. Conclusions: The optimal ratio that made adipose derived stem cells expressed the maximum cartilage specific extracellular matrix was 1∶5. The co-culture inhibited the hypertrophic problem when compared with growth factor induction.
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Received: 06 November 2015
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