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| Research on the Synthesis of Modified Bacterial Cellulose/Hydroxyapatite Sponges |
| Nan Fang1, Wang Qiaoli2, Lai Chen2, Xi Tingfei1, 2* |
1(School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou 325035,Zhejiang, China)
2(Shenzhen key Laboratory of Human Tissue Regeneration and Repair, Shenzhen Institute of Peking University, Shenzhen 518057,Guangdong, China) |
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Abstract The purpose of this study is to investigate three kinds of preparation methods for bacterial cellulose and hydroxyapatite sponges and compare the microstructure and characteristics.The modified bacterial cellulose (TBC) and hydroxyapatite (HA) sponges were fabricated by in situ formation, physical mixing orbiomineralization. Samples prepared by the different methods were characterized using scanning electron microscopy (SEM), energy spectrum analysis(EDX),X-ray diffraction (XRD), Fourier IR transform spectroscopy (ATR-FTI). Furthermore mechanical performance of the sponges prepared with different parameters were tested as well.Experimental results showed that by thein situ formation, physical mixing orbiomineralization HA was successfully deposited on TBC nanofibers, but mechanisms were different.By the in situ formation method, HA nanoparticles took the form of chelate keys associated with TBC nano fibers on the carboxyl. In the physical mixing orbiomineralization methods, HA nanoparticles became electrostatically adsorbed on TBC nanofibers.XRD results showed that there were obvious (211) peaks in the scaffolds synthesized by different methods, but there were significant differences in the morphology of the peaks.Mechanical results showed that microstructure and mechanical properties of sponge also had very big difference with different composite methods. By the in situ formation method, the scaffold minimum intensity was reduced from 4.67MPa to 1.00MPa,while by the biomineralization, scaffold maximum intensitywas increased from 4.67MPa to 5.55MPa. Through the analysis of the microstructure and characterization of the scaffolds,our study could provide the basis for their application to bone tissue engineering.
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Received: 25 September 2015
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