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| Development of HighSpeed Medical Electrolyte Analyzer Based on Flow Injection |
1(Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518000, China
2 Shenzhen New Industries Biomedical Engineering Co., Ltd, Shenzhen 518000, China |
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Abstract To meet the demand of automatic biochemical analyzer electrolyte test, a flow injection dynamic ion analysis method was proposed based on method and experiments in this paper. The new method took advantages of flow injection analysis (FIA) and ion selective electrode (ISE). With embedded technology, we developed a new electrolyte module and tested its accuracy, precision, stability and cross contamination. Experiment results showed that the relative deviation and cross contamination of K+、Na+ and Cl- were under 1%; the coefficient of variability respectively was 0.40%, 0.22% and 0.66% respectively; linearity error was -1.10%，-0.68% and -0.46% respectively; And stability was 1.11%, 0.76% and 1.39% respectively. The clinical results showed that the electrolyte module met the requirement of the clinical application. Compared with conventional electrolyte analyzer, this module can be applied together with automatic biochemical analysis instrument; moreover, its test speed reached 150 samples/h, which allows it to be used in the field of highspeed electrolyte analysis
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［1］罗浔阳,张劼,孙兵，等.日立7600-020E生化分析仪电解质模块应用评价［J］.医疗设备信息,2007,22(5):53-54.
［2］张会生.HC9886型全自动多参数电解质分析仪的研制［J］.现代科学仪器,2004,(2):26-29.
［3］王拥军.两种分析仪测定血清电解质的方法学对比与评估 ［J］.齐齐哈尔医学院学报,2008,29(17):2063-2064.
［4］宋延荣,张萍.基层医院生化分析仪使用情况调查及对策 ［J］.实用医技杂志,2011,18(11):1024.
［5］刘玮,刘克芹.日立7600-110生化分析仪电解质模块应用评价［J］.河北北方学院学报,2010,27(1):48-50.
［6］朱世银,刘玲.日立生化分析仪H,D液的研制与应用［J］.临床军医杂志,2001,29(2):124.
［7］National Committee for Clinical Laboratory Standard. Evaluation of the linearity of quantitative measurement procedures: a statistical approach. Approved Guideline［S］.NCCLS document EP6A.Wayne, Pennsylvania:NCCLS,2003,1(100):9-47.
［8］National Committee for Clinical Laboratory Standard.Method comparison and bias estimation using patient samples ［S］. Approved Guideline,EP9A,Pennsylvania,1995,15(7):1-2.
［9］金绍祥.流动注射分析法与多种仪器分析联用的进展［J］.理化检验-化学分册,2009,45(2):238-240.
［10］龙波,吕薇,陈梨春.日立7180E生化分析仪电解质模块性能评价指标的研究［J］.激光杂志,2011，32(4):96-97.
［11］Ruzicka J,Hansen EH. Flow Injection Analysis ［M］.（2nd Edition）. New York: John Wiley & Sons,1988.
［12］方肇伦.流动注射分析法［M］.北京:科学出版社,1999.
［13］Ruzicka J,Hansen EH.. Flow Injection Analysis ［M］.New York: John Wiley&Sons,1981.
［14］Ukeda H. FIA Bibliography ［J］. Flow Injection Anal,2004,(21):69-79.
［15］董慧如.流动注射分析的原理及影响因素［J］.化学通报,1987, (3):34-79.
［16］杨根元.使用仪器分析［M］.北京:北京大学出版社,2001.
［17］崔云龙.酶法测定血清碳酸氢盐［J］.临床检验杂志,1993,11(3):134-135.
［18］Alexander PW, Dimitrakopoulos T, Hibert DB.Operational assessment of a protentiometric eightsensor f
low cell in a portable flow injection analyzer［J］. Field Analytical Chemistry And Technology,1996, 1(1):31-37.
［19］Martinotti V,Balordi M, Ciceri G. A flow injection analyser conductometric coupled system for the field analysis of free dissolved CO2 and total dissolved inorganic carbon in natural waters ［J］. Anal Bioanal Chem,2012,403:1083-1093.
［20］Li Qingyang, Morris KJ, Dasgupta PK. Partable flowinjection analyzer with liquidcore waveguide based fluorescence, luminescence, and long path length absorbance detector ［J］. Analytica Chimica Acta,2003,479:151-165.
［21］Han Bin, Cao Lei. Flow injectionspectrophotometric determination of total dissolved nitrogen in seawater based on quantificational solenoid valves ［J］.SpectrosCopy and Spectral Analysis，2012,32(1):219-222.
［22］Wehe CA, Bornhorst J, Holtkamp M. Fast and low sample consuming quantification of manganese in cell nutrient solutions by flow injection ICPQMS ［J］. Metallomi,2011,3(12):1291-1296.
［23］Mao Lisha, Chen Huiling, Liu Guihua. Flow injection analysis method for hygienic examination of volatile phenol compounds in the air of residential area ［J］. Journal of hygiene Research, 2011, 40(6):773-775.
［24］叶华俊,夏阿林,张学锋,等.基于长波近红外的便携式品质分析仪的研制及应用［J］.仪器仪表学报,2012,33(1):86-89.
［25］杨邱青,朱智甲,赵爱东.直管型智能流动注射化学发光分析仪的研制［J］.仪器仪表学报,2005,26(10):1024-1026.
［26］黄志敏,李科杰,许海平.流动注射式水质在线测控系统的研究［J］.仪器仪表学报,2005,26(4):344-346.
［27］刘伯强,马登程,王明时,等.一种基于DSP结构的电解质分析仪的设计与实现［J］.仪器仪表学报,2006,27(4):410-412.
［28］Atia MJ, Zeng Jiang. An explicit foumula for the linearization coefficients of Bessel polynomials ［J］. Ramanujan Journal, 2012,28(4):211-221.
［29］杨昌国.精密度评价和方法比较中NCCLS评价方案的应用［J］.临床检验杂志,1993(1):47-49.
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