人E 钙粘素融合蛋白基质对人骨髓间充质干细胞向肝细胞定向诱导分化的影响研究

doi:10.3969/j.issn.0258-8021. 2013. 06.010

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Abstract Hepatocytes differentiating from human mesenchymal stem cells (hBMSCs) hold great potentials for cellbased approaches to liver diseases and drug development. In order to effectively expand and induce hepatic differentiation of hBMSCs, a fusion protein consisting of human E-cadherin extracellular domain and the immunoglobulin G Fc region (hE-cadherin-Fc) was biosynthesized and used as an extracellular matrix biomimicking epithelia cell adhesion junction. The effects of the hE-cadherin-Fc matrix on the hepatic differentiation of hBMSCs were studied in this paper. Compared with TC-PS and gelatin coated surfaces, the hE-cadherin-Fc matrix effectively stimulated the hBMSCs to progress toward the polygonal morphology of hepatocyte, and enhanced the differentiated cells expressing hepatocytespecific genes and livespecific functions. These results show hE-cadherin-Fc is a promising artificial extracellular matrix (ECM) for the hBMSCs differentiation via homophilic interaction of hE-cadherin and synergy between cell adhesion molecular and growth factors.

Key words： human mesenchymal stem cells hepatocytes differentiation fusion protein hE-cadherin

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	XU Jian Bin¹ZHANG Yan¹LI Su Hua¹ZHU Chuan Shun¹
	2YAO Fang Lian²TOSHIHIRO Akaike^{3 &n}

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XU Jian Bin¹ZHANG Yan¹LI Su Hua¹ZHU Chuan Shun^1,2YAO Fang Lian²TOSHIHIRO Akaike^{3 &n. Human ECadherinFc Fusion Protein Matrix Improving the Hepatic Differentiation of Human Mesenchymal Stem Cells[J]. journal1, 2013, 32(6): 708-715.}

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http://cjbme.csbme.org/EN/ 10.3969/j.issn.0258-8021. 2013. 06.010 OR http://cjbme.csbme.org/EN/Y2013/V32/I6/708

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