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Human ECadherinFc Fusion Protein Matrix Improving the Hepatic Differentiation of Human Mesenchymal Stem Cells |
1 College of Life Sciences, Nankai University, Tianjin 300071, China
2 School of Chemical Engineering, Tianjin University, Tianjin 300072, China
3 Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama 226-8501, Japan |
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Abstract Hepatocytes differentiating from human mesenchymal stem cells (hBMSCs) hold great potentials for cellbased approaches to liver diseases and drug development. In order to effectively expand and induce hepatic differentiation of hBMSCs, a fusion protein consisting of human E-cadherin extracellular domain and the immunoglobulin G Fc region (hE-cadherin-Fc) was biosynthesized and used as an extracellular matrix biomimicking epithelia cell adhesion junction. The effects of the hE-cadherin-Fc matrix on the hepatic differentiation of hBMSCs were studied in this paper. Compared with TC-PS and gelatin coated surfaces, the hE-cadherin-Fc matrix effectively stimulated the hBMSCs to progress toward the polygonal morphology of hepatocyte, and enhanced the differentiated cells expressing hepatocytespecific genes and livespecific functions. These results show hE-cadherin-Fc is a promising artificial extracellular matrix (ECM) for the hBMSCs differentiation via homophilic interaction of hE-cadherin and synergy between cell adhesion molecular and growth factors.
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