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| Advances in Microfluidic Sensor Chip Analysis of Tumor-Derived Exosomes |
| Yang Chuang1,2 , He Hong1,2, Ge Chuang1,3, Xu Yi1,2* |
1(Key Subject Laboratory of Novel Micro and Nano Devices and System Technology, Chongqing University & Key Laboratory of Optoelectronics Technology and System, Ministry of Education, Chongqing University, Chongqing 400044, China)
2(School of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China)
3(Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing 400030, China) |
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Abstract Exosomes are nanoscale vesicles secreted by a variety of cells and carry biomarkers such as nucleic acids, proteins and lipids. They not only participate in the exchange and transmission of information between cells but also play important roles in various physiological and pathological activities of the human body. Therefore, exosome detection is of great value for the early diagnosis, drug therapy and prognosis evaluation of tumors. Microfluidic analysis has become a powerful tool for exosome enrichment and detection because of its good integration, high sensitivity and fast analysis speed. This paper focused on introducing exosome separation and enrichment technologies including immunosorbent chips, nanostructured filter chips, lateral deterministic displacement separation chips, dielectrophoresis separation chips, and swimming separation chips. Microfluidic chips that integrate fluorescence, electrochemistry, surface enhanced Raman scattering and other detection technologies including microstructure and new materials were also introduced. The advantages of microfluidic analysis techniques were compared with that of conventional methods. In view of the difficulties and challenges in the accumulation and identification of exosomes in complex samples, the latest research progress of tumor-derived exosomes detection technology based on microfluidic analysis technology was reviewed, and the future development trend of microfluidic chips for the detection of exosomes with high sensitivity and efficiency was discussed and prospected.
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Received: 30 April 2024
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