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Abstract Drug-induced liver injury (DILI) is an important part of drug safety evaluation. Existing evaluation methods mainly rely on in vitro two-dimensional cell culture models and animal models, which cannot simulate the human complex physiological environment and have limited predictive ability for drug metabolism and toxicity. Therefore, it is necessary to develop in vitro liver replacement models that can accurately reproduce normal functions of human liver and its responses to drug toxicity. Human liver-on-chips not only have more comprehensive physiological microstructures and functions than two-dimensional cell models, but also eliminate species differences between animals and humans, and more effectively simulate the physiological or pathological state of human liver. At present, the human liver-on-chip, as a novel in vitro anthropomorphic model for DILI detection, is still in the initial development stage and has not yet formed a unified standard or guideline. However, its enormous potential and application prospects have attracted widespread attention. This consensus is oriented to the evaluation of DILI, relying on the human liver-on-chip platform, combined with relevant domestic and foreign model construction schemes, toxicity detection indicators and drug lists, as well as research literatures and data, to form this expert consensus and provide relevant suggestions. The consensus is aiming to promote the development of human liver-on-chip technology and its application in the detection of DILI.
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Received: 20 December 2023
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Corresponding Authors:
*E-mail: gu@seu.edu.cn;zhangjuan@seu.edu.cn
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