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The Role of Cochlear Auditory Pathways on Motor CorticalActivation of Transcranial Magneto-Acoustic Stimulation |
Zhou Xiaoqing1#, Liu Ruixu1, Tan Ruxin1, Wang Huiqin1, Yin Tao1,2#, Liu Zhipeng1#* |
1(Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China) 2(Neuroscience Center, Chinese Academy of Medical Sciences, Beijing 100730, China) |
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Abstract Transcranial magneto-acoustic stimulation (TMAS) technique can noninvasively deliver millimeter-scale precision electrical stimulation of the whole brain, including the deep brain regions. Existing experimental results have preliminarily confirmed that TMAS is a composite physical stimulation containing coupled MA electric field and ultrasonic field simultaneously. Recent research results show that cochlear auditory pathway is a necessary condition for cortical activation by focused ultrasound. In this paper, in vivo TMAS and TUS were contrastively stimulated in deaf model mice with damaged auditory pathway for the first time to analyze the role of auditory pathways in the neuromodulation of TMAS. Electromyography (EMG) signals and motor feedback induced by TUS and TMAS were collected from deaf mice (n=6) and normal mice (as the control group, n=6) at the different time points after the deaf-model building. The different neuromodulation effects of two kinds of stimulations on the motor cortex of deaf mice and normal mice were compared and analyzed. Results showed that: At 1 h, 12 h, 24 h and 48 h after model building, the motor feedback of deaf mice under TMAS and TUS gradually disappeared, and the success rate of EMG decreased to 3.33%; Normal mice at the same time point could produce motor feedback and EMG under the two kinds of stimulations, and there was no statistical difference in the success rate of EMG between the TUS group and the TMAS group (p=0.296); but the root mean square amplitude of EMG in the TMAS group was greater than that in the TUS group, and the difference was statistically significant (p=0.0011). The results indicated that the stimulation effects of both TMAS and TUS were heavily dependent on the integrity of auditory pathways. By comparing the results of TUS stimulation between the deaf group and the normal group, it was verified that the existence of auditory pathway was a necessary condition for ultrasound neuromodulation during motor cortex stimulation; the stimulation effect of TMAS was mainly dependent on the stimulation effect of ultrasonic field, and the auditory pathway also had a decisive influence for neuromodulation with TMAS. It is further suggested that the MA electric field in TMAS promote the influence for neuromodulation by TUS in motor cortex stimulation.
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Received: 11 January 2021
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About author:: Member, Chinese Society of Biomedical Engineering |
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