脱细胞技术在组织工程血管中的应用进展

doi:10.3969/j.issn.0258-8021.2021.01.13

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Abstract The decellularized blood vessel prepared by decellularizing the natural blood vessel is considered as one important kind of tissue engineering vascular scaffolds and has broad application prospects. However, the preparation of extracellular matrix (ECM) still lacks uniform standards. The choice of decellularization methods depends on the tissue source and the application of the ECM. The decellularization protocols used for preparing ECM are very important, especially for the ECM scaffolds such as decellularized blood vessels that need to withstand the blood press for a long time. The maintenance of ECM integrity and cell removal efficiency are highly dependent on the specific approaches used for decellularization. This article reviewed decellularization methods applied to blood vessels, including physical, chemical, enzymatic and serum applications in the preparation of decellularized blood vessels. The decellularization efficiency of different decellularization methods and their effects on the tissue structure, mechanical properties and biological activity of the vascular matrix were compared, and the importance of developing reasonable decellularization protocols was emphasized.

Key words： decellularization extracellular matrix blood vessel scaffolds tissue engineering

Received: 02 April 2020

PACS:

R318

Corresponding Authors: *E-mail:Yongquan_Gu@163.com

About author:: #（Member, Chinese Society of Biomedical Engineering）

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	Cheng Jin
	Wang Cong
	Gu Yongquan

Cite this article:

Cheng Jin,Wang Cong,Gu Yongquan. Progress on the Application of Decellularization Techniques in Tissue Engineering Vascular Scaffolds[J]. Chinese Journal of Biomedical Engineering, 2021, 40(1): 118-123.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2021.01.13 OR http://cjbme.csbme.org/EN/Y2021/V40/I1/118

[1] Matsuzaki Y, John K, Shoji T, et al. The evolution of tissue engineered vascular graft technologies: From preclinical trials to advancing patient care [J]. Appl Sci (Basel),2019,9(7): 1274.
[2] Carrabba M, Madeddu P. Current strategies for the manufacture of small size tissue engineering vascular grafts [J]. Front Bioeng Biotechnol, 2018, 6:41.
[3] Yuan Haoyong, Chen Chunyang, Liu Yuhong, et al. Strategies in cell-free tissue-engineered vascular grafts [J]. J Biomed Mater Res A, 2020, 108(3): 426-445.
[4] Langer R, Vacanti JP. Tissue engineering [J]. Science, 1993, 260(5110): 920-926.
[5] Liu Xiaohong, Cai Yang, Xia Cuiping, et al. An innovative method to obtain porous porcine aorta scaffolds for tissue engineering [J]. Artif Organs, 2019, 43(12): 1162-1169.
[6] Daniel J, Abe K, McFetridge PS. Development of the human umbilical vein scaffold for cardiovascular tissue engineering applications [J]. ASAIO Journal, 2005, 51(3): 252-261.
[7] Funamoto S, Nam K, Kimura T, et al. The use of high-hydrostatic pressure treatment to decellularize blood vessels [J]. Biomaterials, 2010, 31(13): 3590-3595.
[8] Mahara A, Morimoto N, Sakuma T, et al. Complete cell killing by applying high hydrostatic pressure for acellular vascular graft preparation [J]. Biomed Res Int, 2014, 2014: 379607.
[9] Negishi J, Funamoto S, Kimura T, et al. Porcine radial artery decellularization by high hydrostatic pressure [J]. J Tissue Eng Regen Med, 2015, 9(11): E144-E151.
[10] Krasilnikova AA, Sergeevichev DS, Fomenko VV, et al. Globular chitosan treatment of bovine jugular veins: Evidence of anticalcification efficacy in the subcutaneous rat model [J]. Cardiovasc Pathol, 2018, 32:1-7.
[11] Jeinsen N, Magel L, Jonigk D, et al. Biocompatibility of intensified decellularized equine carotid arteries in a rat subcutaneous implantation model and in a human in vitro model [J]. Tissue Eng Part A, 2018, 24(3-4):310-321.
[12] Fitriatul N, Sha'ban M, Azhim A. Evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment [J]. Conf Proc IEEE Eng Med Biol Soc, 2017, 2017:2072-2075.
[13] Azhim A. The use of sonication treatment to completely decellularize blood arteries: a pilot study [C] // Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Boston: IEEE, 2011: 2468-2471.
[14] Sakakibara S, Ishida Y, Hashikawa K, et al. Intima/medulla reconstruction and vascular contraction–relaxation recovery for acellular small diameter vessels prepared by hyperosmotic electrolyte solution treatment [J]. J Artif Organs, 2014, 17(2): 169-177.
[15] Tondreau MY, Laterreur V, Gauvin R, et al. Mechanical properties of endothelialized fibroblast-derived vascular scaffolds stimulated in a bioreactor [J]. Acta Biomater, 2015, 18: 176-185.
[16] Lin CH, Hsia K, Tsai CH, et al. Decellularized porcine coronary artery with adipose stem cells for vascular tissue engineering [J]. Biomed Mater, 2019, 14(4): 045014.
[17] Sheridan WS, Ryan AJ, Duffy GP, et al. An experimental investigation of the effect of mechanical and biochemical stimuli on cell migration within a decellularized vascular construct [J]. Ann Biomed Eng, 2014, 42(10): 2029-2038.
[18] Mangold S, Schrammel S, Huber G, et al. Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering - comparison with endothelium-denuded HUV [J]. J Tissue Eng Regen Med, 2015, 9(1): 13-23.
[19] van Steenberghe M, Schubert T, Bouzin C, et al. Decellularized and secured porcine arteries with naoh-based process: Proof of concept [J]. Ann Vasc Surg, 2018, 49: 179-190.
[20] Sheridan WS, Duffy GP, Murphy BP. Mechanical characterization of a customized decellularized scaffold for vascular tissue engineering [J]. J Mech Behav Biomed Mater, 2012, 8: 58-70.
[21] Chaparro FJ, Matusicky ME, Allen MJ, et al. Biomimetic microstructural reorganization during suture retention strength evaluation of electrospun vascular scaffolds [J]. J Biomed Mater Res B Appl Biomater, 2016, 104(8): 1525-1534.
[22] Dahl SLM, Koh J, Prabhakar V, et al. Decellularized native and engineered arterial scaffolds for transplantation [J]. Cell Transplant, 2003, 12(6): 659-666.
[23] Zou Yu, Zhang Yanhang. Mechanical evaluation of decellularized porcine thoracic aorta [J]. J Surg Res, 2012, 175(2): 359-368.
[24] Simsa R, Padma AM, Heher P, et al. Systematic in vitro comparison of decellularization protocols for blood vessels [J]. PLoS ONE, 2018, 13(12): e0209269.
[25] Bertanha M, Sobreira ML, Bovolato ALC, et al. Ultrastructural analysis and residual DNA evaluation of rabbit vein scaffold [J]. Acta Cir Bras, 2017, 32(9): 706-711.
[26] Lin CH, Lu JH, Hsia K, et al. The Antithrombotic function of sphingosine-1-phosphate on human adipose-stem-cell-recellularized tissue engineered vascular graft in vitro [J]. Int J Mol Sci, 2019, 20: 5218.
[27] Mancuso L, Gualerzi A, Boschetti F, et al. Decellularized ovine arteries as small-diameter vascular grafts [J]. Biomed Mater, 2014, 9(4): 045011.
[28] Tuan-Mu HY, Yu CH, Hu JJ. On the decellularization of fresh or frozen human umbilical arteries: implications for small-diameter tissue engineered vascular grafts [J]. Ann Biomed Eng, 2014, 42(6): 1305-1318.
[29] Cheng Jin, Wang Cong, Gu Yongquan. Combination of freeze-thaw with detergents: A promising approach to the decellularization of porcine carotid arteries [J]. Biomed Mater Eng, 2019, 30(2): 191-205.
[30] Hazwani A, Sha'Ban M, Azhim A. Characterization and in vivo study of decellularized aortic scaffolds using closed sonication system [J]. Organogenesis, 2019, 15(4): 120-136.
[31] Theodoridis K, Muller J, Ramm R, et al. Effects of combined cryopreservation and decellularization on the biomechanical, structural and biochemical properties of porcine pulmonary heart valves [J]. Acta Biomater, 2016, 43: 71-77.
[32] Dimitrievska S, Cai C, Weyers A, et al. Click-coated, heparinized, decellularized vascular grafts [J]. Acta Biomater, 2015, 13: 177-187.
[33] Bai Hualong, Dardik A, Xing Ying. Decellularized carotid artery functions as an arteriovenous graft [J]. J Surg Res, 2019, 234: 33-39.
[34] Walawalkar S, Almelkar S. Fabrication of aortic bioprosthesis by decellularization, fibrin glue coating and re-endothelization: a cell scaffold approach [J]. Prog Biomater, 2019, 8(3): 197-210.
[35] Van de Walle AB, Uzarski JS, McFetridge PS. The consequence of biologic graft processing on blood interface biocompatibility and mechanics [J]. Cardiovasc Eng Technol, 2015, 6(3): 303-313.
[36] McFetridge PS, Daniel JW, Bodamyali T, et al. Preparation of porcine carotid arteries for vascular tissue engineering applications [J]. J Biomed Mater Res A, 2004, 70(2): 224-234.
[37] Uchimura E, Sawa Y, Taketani S, et al. Novel method of preparing acellular cardiovascular grafts by decellularization with poly(ethylene glycol) [J]. J Biomed Mater Res A, 2003, 67(3): 834-837.
[38] Xu Shuangyue, Lu Fangna, Cheng Lianna, et al. Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model [J]. Biomed Eng Online, 2017, 16(1): 55.
[39] Yang Daping, Guo Tiefang, Nie Chunlei, et al. Tissue-engineered blood vessel graft produced by self-derived cells and allogenic acellular matrix: a functional performance and histologic study [J]. Ann Plast Surg, 2009, 62(3): 297-303.
[40] Liu Guofeng, He Zhijuan, Yang Daping, et al. Decellularized aorta of fetal pigs as a potential scaffold for small diameter tissue engineered vascular graft [J]. Chin Med J (Engl), 2008, 121(15): 1398-1406.
[41] Lin CH, Kao YC, Ma H, et al. An investigation on the correlation between the mechanical property change and the alterations in composition and microstructure of a porcine vascular tissue underwent trypsin-based decellularization treatment [J]. J Mech Behav Biomed Mater, 2018, 86: 199-207.
[42] Perea-Gil I, Uriarte JJ, Prat-Vidal C, et al. In vitro comparative study of two decellularization protocols in search of an optimal myocardial scaffold for recellularization [J]. Am J Transl Res, 2015, 7(3): 558-573.
[43] Gilpin A, Yang Y. Decellularization strategies for regenerative medicine: From processing techniques to applications [J]. Biomed Res Int, 2017, 2017: 9831534.
[44] Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes [J]. Biomaterials, 2011, 32(12): 3233-3243.
[45] Ishino N, Fujisato T. Decellularization of porcine carotid by the recipient's serum and evaluation of its biocompatibility using a rat autograft model [J]. J Artif Organs, 2015, 18(2): 136-142.
[46] Katsimpoulas M, Morticelli L, Gontika I, et al. Biocompatibility and immunogenicity of decellularized allogeneic aorta in the orthotopic rat model [J]. Tissue Eng Part A, 2019, 25(5-6): 399-415.
[47] Lindsey P, Echeverria A, Cheung M, et al. Lower extremity bypass using bovine carotid artery graft (artegraft): An analysis of 124 cases with long-term results [J]. World J Surg, 2018, 42(1): 295-301.