微流控法低温保护剂添加及去除线型优化研究

doi:10.3969/j.issn.0258-8021.2019.06.010

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Abstract Cryoprotectants (CPA) addition and removal are essential steps in oocytes cryopreservation. In recent years, microfluidic chips have been used for addition and removal of CPA. The way of addition and removal in microfluidic chips will affect the survival rate and in vitro development of oocytes. In this paper, linear, convex and concave CPA loading or removal protocols were realized by adjusting injection models of microinjection pumps. Nine addition-removal combined protocols were achieved in the microfluidic system. The survival rate and in vitro development of oocytes with different CPA addition-removal protocols were investigated. Results showed that the survival rate (97.22%) and morula rate (46.03%) of porcine MII oocytes from concave loading-convex unloading protocol were significantly higher than that using other protocols(P < 0.05). There was a certain matching relationship between the addition and removal of CPA. This research provided a new solution for optimizing continuous addition-removal of CPA by microfluidic method.

Key words： cryoprotectants microfluidic chip oocytes optimization

Received: 03 May 2018

PACS:

R318

Corresponding Authors: E-mail: zjulily@163.com

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	Articles by authors
	Zhou Xinli
	Du Yukun
	Yi Xingyue
	Dai Jianjun
	Zhang Defu

Cite this article:

Zhou Xinli,Du Yukun,Yi Xingyue, et al. Optimization of Cryoprotectants Addition and Removal Protocols Using Microfluidic Chip[J]. Chinese Journal of Biomedical Engineering, 2019, 38(6): 719-725.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2019.06.010 OR http://cjbme.csbme.org/EN/Y2019/V38/I6/719

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