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| A Novel Method for Cell Distribution Using an Integrated Superhydrophobic Microwell Array Chip |
| Jiao Pengcheng1#, Zhao Tian2#, Ji Jiaojiao1, Yu Bin1, Wu Rong1, Liu Peng2*, Feng Qianqian1* |
1(Center of Biomedical Analysis, School of Life Sciences, Tsinghua University, Beijing 100084, China)
2(School of Biomedical Engineering, Tsinghua University, Beijing 100084, China) |
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[1] Yushuai W, Kaiyi L, Yaqian L, et al.Grouped-seq for integrated phenotypic and transcriptomic screening of patient-derived tumor organoids[J].Nucleic Acids Research, 2021,50(5): e28.
[2] Zhang PF, Zhang JX, Bian ST, et al.High-throughput superhydrophobic microwell arrays for investigating multifactorial stem cell niches[J].Lab on a Chip, 2016.16(16):2996-3006.
[3] Hu Y, Sui X, Song F, et al.Lung cancer organoids analyzed on microwell arrays predict drug responses of patients within a week[J].Nature Communications, 2021, 12(1):2581.
[4] Liu C, Li K, Sui X, et al.Patient-derived tumor organoids combined with function-associated ScRNA-Seq for dissecting the local immune response of lung cancer[J].Advanced Science, 2024, 11: e2400185.
[5] Zhao T, Lei Y, Liu C, et al.A Versatile high-throughput single-cell screening platform for profiling antigen-specific long-lived b cells in blood and bone marrow[J].Advanced Science, 2025,12: e2414945.
[6] Kim M, Mun H, Sun CO, et al.Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening[J].Nature Communications, 2019, 10(1): 3991.
[7] Arnold LW, Lannigan J.Practical issues in high-speed cell sorting[J].Curr Protoc Cytom, 2010, 51(1).
[8] Maecker HT, Frey T, Nomura LE, et al.Selecting fluorochrome conjugates for maximum sensitivity[J].Cytometry Part A : The Journal of the International Society for Analytical Cytology, 2004, 62(2): 169-173.
[9] Wang SR, Zhong N, Zhang X M, et al.OMIP 071: a 31-parameter flow cytometry panel for in-depth immunophenotyping of human T-cell subsets using surface markers[J].Cytometry Part A : The Journal of the International Society for Analytical Cytology, 2021, 99(3): 273-277.
[10] Ibrahim SF, Van Den EG.Flow cytometry and cell sorting[J].Advances in Biochemical Engineering/Biotechnology, 2007, 106: 19-39.
[11] Jensen HA, Wnek R.Analytical performance of a 25-marker spectral cytometry immune monitoring assay in peripheral blood[J].Cytometry,2021, 99:180-193.
[12] Solomon M, Delay M, Reynaud D.Phenotypic analysis of the mouse hematopoietic hierarchy using spectral cytometry: from stem cell subsets to early progenitor compartments[J].Cytometry Part A : The Journal of the International Society for Analytical Cytology, 2020, 97(10): 1057-1065.
[13] Si J, Shi X, Sun S, et al.Hematopoietic progenitor kinase1 (HPK1) mediates T cell dysfunction and is a druggable target for T cell-based immunotherapies[J].Cancer Cell, 2020, 38(4): 551-566.
[14] Cossarizza A, Chang HD, Radbruch A, at al.Guidelines for the use of flow cytometry and cell sorting in immunological studies[J].European Journal of Immunology, 2017, 47(10):1584-1797.
[15] Zigon ES, Purseglove SM, Toxavivis V, et al.A rapid single cell sorting verification method using plate-based image cytometry[J].Cytometry Part A: The Journal of the International Society for Analytical Cytology, 2018, 93(10): 1060-1065. |
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