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| Characterization of Cell Activity Distribution in Tissue Engineering Based on Scattering OCT |
| Lu Ming1, Wang Ling1,2*, Yang Shanshan1,2, Xu Mingen1,2 |
1(School of Automation, Hangzhou Dianzi University, Hangzhou 310018, China)
2(Zhejiang Provincial Key Lab of Medical Information and Three-Dimensional Bio-Printing,Hangzhou 310018, China) |
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Abstract Non-invasive and label-free detection of cell activity distribution in tissue engineering is of great significance. This paper proposed a scattering optical coherence tomography (OCT) technology to quantify and characterizing the cell activity distribution in a three-dimensional tissue model, and proposed an optimized depth-resolved (ODR) scattering algorithm to reconstruct the cell/material scatter contrast enhanced images, achieving the quantitative analysis of the correlation between scattering coefficients, cell concentration and activity status, and characterize the cell activity distribution in three-dimensional tissues. The experimental results of multi-layer sample model showed that the ODR scattering algorithm was able to improve the sensitivity and depth of sample scattering imaging. The human hepatocellular carcinoma cell C3A and human dermal fibroblast cell-laden hydrogel were used to verify that the cell concentration and cell activity in the material are linearly correlated with the scattering coefficient (92.07%) and Pearson correlation coefficient (99.13%) respectively, and the scattering coefficient could be used to quantify the cell concentration and survival status in the hydrogel scaffold. ODR-based scattering OCT realized the non-invasive and label-free detect of cell activity distribution in tissue engineering scaffolds, allowing to carry out long-term research on the cell activity distribution of cell-laden scaffolds, therefore, having potentials of a powerful monitoring tool for disease model construction, drug screening and cell therapy.
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Received: 08 November 2021
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Corresponding Authors:
*E-mail: lingw@hdu.edu.cn
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