基于细胞3D打印技术的肿瘤药物筛选细胞芯片研究

doi:10.3969/j.issn.0258-8021. 2014. 02.005

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Abstract There are some crucial problems in conventional drug screening systems. For example, animal models have shortcomings of species differences and long test periods; in addition, poor mimics of in vivo microenvironment results in the low accuracy of cellbased highthroughput drug screening assays. The 3D cell printing technology may provide solutions of establishing biomimetic tissue models in vitro. Moreover, the combination of 3D cell printing and cell chip provides a highly valuable tool for the efficient and accurate drug screening in vitro. In this study, a cell chip integrating several groups of IDEs, was designed and manufactured. Then, the HMSCH and HO-8910 cell lines were assembled within the gelatin/alginate mixture on the manufactured chip using 3D cell printing. The IDEs was used to detect changes in the cell impedance which reflects the cell growth, adhesion, proliferation, apoptosis and effects of drugs on the viability. Based on this cellsloaded chip, the antitumor effect or hepatoxicity of both CTX and DDP were measured respectively. Our results showed that the 3D structure could suppot the growth of HMSC-H or HO-8910 cells. The scaffold possessed pores with diameter of 200-300 μm. The change of impedance caused by the cell proliferation was precisely detected by the chip at 10.4 Hz using DMEM as electrolyte solution, 20 h later, the cell impedance increased by 69.6%. Using this chipbased in vitro drug screening model, we simultaneously detected the antitumor effect and hepatotoxicity of the drugs. Meanwhile, the efficacy of these drugs, which require hepatic metabolism for activation such as CTX, was also evaluated in the same chip.

Key words： 3D cell printing cell chip drug screening IDEs scaffold

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	ZHAO Zhan Ying XU MingEn^* SHI Ran GUO Miao YAN Ming XU Ying WANG Ling

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ZHAO Zhan Ying XU MingEn^* SHI Ran GUO Miao YAN Ming XU Ying WANG Ling. Research of AntiTumor Drug Screening Cell Chip Based on 3D Cell Printing Technique[J]. journal1, 2014, 33(2): 161-169.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021. 2014. 02.005 OR http://cjbme.csbme.org/EN/Y2014/V33/I2/161

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