胶原/纤维蛋白胶/载BSA微球复合支架的制备及体外性能研究

doi:10.3969/j.issn.0258-8021. 2014.01.012

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Abstract In order to overcome the problem of nutrition insufficiency and inhomogeneous distribution in tissue engineering scaffolds, a scaffold (S_CFM) composed of collagen/fibrin/BSA microspheres was prepared and the features were characterized, especially the release behaviors of BSA. Porous collagen scaffolds for tissue engineering were fabricated by freezedrying and crosslinking. BSA loaded microspheres mixed with fibrin into collagen scaffolds for S_CFM. The physiochemical properties of the scaffolds, such as surface morphology, porosity, pore sizes，mechanical function, and BSA contents in the scaffold were measured. SEM results showed that collagen scaffolds exhibited a highly porous and interconnected structure, the pore size of the material was (130±45) μm. Results of in vitro release tests revealed the BSA released from S_CFM was 72.36%±3.48% of the original amount of BSA encapsulated after 144 h. The cumulative release of BSA from collagen/fibrin/BSA scaffolds (S_CFB) within 48 h was 75.20%±2.74 %. There were no significant changes in the further prolonged period after 48 h. The S_CFMachieved a relatively constant release and prolonged BSA release properties as compared to the S_CFB. It was found that BSA concentration inside of S_CFMwas higher than the external release medium before 96 h. The conformations of the BSA from the S_CFM did not have significant changes. The S_CFMobtained sustaining release ability and can maintain a homogeneous concentration of the BSA inside the scaffold for a long time. Therefore it provides a kind of promising scaffolds for tissue engineering.

Key words： collagen fibrin PLGA microspheres tissue engineering scaffolds

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	CHEN Hong Li¹LV Jie Li²NAN Wen Bin¹LIU Rui¹ZHANG Hui^2GUO Wei Yu

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CHEN Hong Li¹LV Jie Li²NAN Wen Bin¹LIU Rui¹ZHANG Hui^2GUO Wei Yu. Preparation and in vitro Characteristics of Collagen/ Fibrin/BSA Microspheres Complex Tissue Engineering Scaffolds[J]. journal1, 2014, 33(1): 79-85.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021. 2014.01.012 OR http://cjbme.csbme.org/EN/Y2014/V33/I1/79

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