基于气动控制的多细胞三维组装技术研究

doi:10.3969/j.issn.0258-8021.2013.02.013

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Abstract Threedimension controlled cellassembly technology provides new theory and technology for tissue engineering and regenerative medicine. Organs have complex threedimensional structures composed of different cells. The multicell assembly technique is one crucial tool to development of cellassembly technology. In this study, we developed a multicell assembly technique based on the pneumatic control. We designed and constructed pneumatic nozzle model by making use of compressed air as power and nonhorizontal arrangement method. Based on this system，we conducted a series of cell assembly experiments on three kinds of color matrix material and two kinds of living cells. Results demonstrated that the system can realize accurate switching and assembly among three nozzles. In the assembling process，jet of nozzle responds quickly at startstop, moreover, the startstop response delay is short than 5 ms and the forming precision is small than 30 μm, the positions of nozzle is accurate, nozzles were not interfere with others, and the system is easy to realize and convenient for multinozzle extend and control. Based on the system, the molding assembly of many kinds of important biological value structure of three kinds of materials, such as ladder structure, circular cavity structure and Polygonal structure can be completed. On the other hand, the assembly of two living cells can also be completed. The results of CD34 and Oil red O staining experiment showed that the growth of the assembly of cells is good within the structures, cellular localization accurate and cell trait keep good. This system is simple, flexible, reliable, easy to realize and convenient for multicell assembly, which can be used in fabricating complex tissue and organs.

Key words： pneumatic nozzle multicell assembly tissue engineering regenerative medicine

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	YANG Min XU Ming En^*CHEN Jie Yi ZHAO Zhan Ying GE Ya Kun YAN Ming GUO Miao

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YANG Min XU Ming En^*CHEN Jie Yi ZHAO Zhan Ying GE Ya Kun YAN Ming GUO Miao
. Research on ThreeDimension MultiCell Assembly Technique Based on Pneumatic Control[J]. journal1, 2013, 32(2): 211-219.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2013.02.013 OR http://cjbme.csbme.org/EN/Y2013/V32/I2/211

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