微囊化对HepG2 细胞渗透压调节基因表达影响及调控研究

doi:10.3969/j.issn.0258-8021.2013.01.010

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Abstract The aim of this work is to investigate the influence of microencapsulation on the expression of the osmoregulation genes and exogenous regulation in HepG2 cells. We compared the expression of sodium/myoinositol cotransporter (SMIT) and taurine transporter (TAUT) in HepG2 cells under different cultured conditions through realtime RTPCR. The effects of exogenous antiosmotic stress matter on cell viability and albumin level were also evaluated through MTT assay and ELISA assay. We did not find a significant difference in the expression of SMIT. However, the results showed that the expression level of TAUT in encapsulated cells was approximately 4.3 times higher than that of monolayer cells（P<0.01）. Accordingly, we found that taurine at 1～1.5 mM significantly increased the viability by 15%～30% and the biosynthetic function by 15% in microencapsulated HepG2 cells（P<0.05）. Trehalose, as for it, did not alter the biosynthetic function but increased the viability of the encapsulated cells by 20%～30% at 0.1 mM（P<0.05）. In conclusion, osmotic stress exists in the microcapsules and affects genes expression. Exogenous antiosmotic stress matter can prevent the inhibition effects of hyperosmotic stress on cellular growth.

Key words： microencapsulated cells sodium/myoinositol cotransporter taurine transporter osmotic stress

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	XIAO Jing¹
	2ZHANG Ying¹YU WeiTing¹WANG Wei¹MA XiaoJun ^1*

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XIAO Jing^1,2ZHANG Ying¹YU WeiTing¹WANG Wei¹MA XiaoJun ^1*
. Effect of Microencapsulation on Expression of Osmoregulation Genes and Exogenous Regulation in HepG2 Cell[J]. journal1, 2013, 32(1): 70-77.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2013.01.010 OR http://cjbme.csbme.org/EN/Y2013/V32/I1/70

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