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| Depthcontrolled and Automated Piezoelectric Micro issection System |
1 Jiangsu Provincial Key Laboratory of Advanced Robotics, Suzhou 215021, China
2 Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, China
3 Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China |
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Abstract In order to obtain a specific population of cells or a single cell from different tissue slice for the research, a depthcontrolled and automated piezoelectric microdissection system was developed to solve problems of time consuming, easy fatigue, and low accuracy in the manualdissection. On the basis of different gray values of foreground and background to detect and track the tip of the dissection needle〖DK〗’s position in rea
ltime, and according to the change of the X axis to judge whether the dissection needle contact the target surface, the depth information was acquired. By analyzing the dissection experiments conducted in different conditions, it is shown that this proposed system can place the needle in any positions of the tissues, quickly scan the whole samples to determine the target area and realize the dissection in specify position with arbitrary shape of biological samples. The fineness of cutting line is 15 μm, the resolution of cutting is 50 nm，and cutting error is 05 μm, reaching the unicellular cut level. In addition, the separation of target tissue sections can be realized automatically. The method provides the improvement of the precision and efficiency of dissections, showing its superiority.
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［1］Arai F, Amano T, Fukuda T, et al. Mechanical microdissection by microknife using ultrasonic vibration and ultra fine touch probe sensor［C］// Proceedings of IEEE International Conference on Robotics and Automation. Seoul: IEEE, 2001, 1: 139-144.
［2］Schuzte K, Becker I, Becker KF. Cut out or poke inthe key to the world of single genes: laser micromanipulationas a valuable tool on the lookout for the origin of disease［J］. Biomolccular Engineering, 1997, 14(1):1-8.
［3］Ichikawa A, Tanikawa T, Akagi S, et al. Automatic cell cutting by highprecision microfluidic control［J］. Journal of Robotics and Mechatronics, 2011,23(1):13-18.
［4］Zhang Yong, Chen BK, Liu Xinyu, et al. Autonomous robotic pickandplace of microobjects ［J］. IEEE Transactions on Robotics, 2010, 26(1):200- 207.
［5］Nath C, Rahman M, Ken SN. Modeling of the effect of machining parameters on maximum thickness of cut in ultrasonic elliptical vibration cutting ［J］. Journal of Manufacturing Science Aand Engineering, 2011, 133:1087-1357.
［6］Gallagher RI, Blakely SR, Liotta LA, et al. Laser capture microdissection: arcturusxt infrared capture and uv cutting methods ［M］//Molecular Profiling: Methods in Molecular Biology. Clifton：Humana Press, 2012:157-178.
［7］Arar F, Morishima K, et al. Biomicromanipulation (New direction for operation improvement) ［C］ // Proceedings of IEEE International Conference on IROS. Grenoble: IEEE, 1997: 1300-1305.
［8］Kim JH. Visually guided 3D micro positioning and alignment system［J］. International Journal of Precision Engineering and Manufacturing, 2011, 12(5): 797-803.
［9］Robert F. Bonner M, Michael EB, et al. Laser capture microdissecion ［J］. Science, 1997, 278::21-22.
［10］杨克己，吴佳杰. 基于超声振动的显微切割技术［J］.工程设计学报，2009,16(1):58-62.
［11］刘亚欣,陈立国,孙立宁,等. 超声振动显微切割工具的设计与研制［J］.光学精密工程,2005,13(增刊): 128-133.
［12］Ru Changhai, Zhang Yong, Sun Yu. Automated fourpoint probe measurement of nanowires inside a scanning electron microscope［J］. IEEE Transactions on Nanotechnology, 2011, 10(4):674-681.
［13］Wang Wenhui, Liu Xinyu, Sun Yu. Contact detection in microrobotic manipulation［J］. The International Journal of Robotics Research, 2007, 26(8): 821-828.
［14］Eichhorn V, Fatikow S, Wich T, et al. Depthdetection methods for microgripper based on the manipulation in a scanning electron microscope［J］. Micro Nano Mechatron, 2008, 4(1):27-36. |
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