Puerarin@FeS2/Ti3C2纳米平台构建及其骨肿瘤术后治疗功能研究

doi:10.3969/j.issn.0258-8021.2026.03.010

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Abstract Addressing the clinical challenge that current osteosarcoma treatment strategies struggle to simultaneously achieve tumor eradication and bone repair, this study designed a multifunctional nanomaterial to synchronously inhibiting osteosarcoma cells and protecting the proliferative activity of osteoblasts, thereby modulating homeostasis to facilitate bone defect repair. FeS₂ nanoparticles with excellent peroxidase-like activity were loaded onto the surface of Ti₃C₂ MXenes, which possess near-infrared (NIR) photothermal response properties, constructing an FeS₂/Ti₃C₂ composite carrier. The substrate was surface-functionalized using amino-polyethylene glycol-hydroxyl (NH₂-PEG-OH). The active herbal component Puerarin was immobilized onto the composite carrier via dithiodiglycolic acid bridging technology, ultimately yielding the Puerarin@FeS₂/Ti₃C₂ nanoplatform. Material characterization techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and Fourier transform infrared spectroscopy (FT-IR) confirmed the successful preparation and composite structural features of the material. Cytotoxicity assays demonstrated that the material exhibited no significant toxicity to mouse fibroblast cells (L929) or macrophage cells (RAW264.7), with cell viability remaining over 80% after long-term co-culture. Under 1 064 nm NIR laser irradiation, the material exerts a photothermal-chemodynamic synergistic response. Compared with the single-stimulus groups, the composite material group exhibits enhanced inhibitory effects on osteosarcoma cell proliferation, with a UMR-106 cell viability of 54.3% after 2 days. At the same time, the introduction of Puerarin exerted a protective effect on BMSCs, showing no significant difference in the proliferation rate compared to the blank control group after 7 days. In conclusion, this work provided a novel strategy for developing integrated nanoplatforms for combined tumor therapy and bone defect repair.

Key words： MXenes chemodynamic therapy (CDT) glutathione peroxidase (GPx) puerarin synergistic osteosarcoma therapy

Received: 09 June 2025

PACS:

R318

Corresponding Authors: ^*E-mail:lisuiyan@home.swjtu.edu.cn;jweng@swjtu.edu.cn

About author:: ^#Member,Chinese Society of Biomedical Engineering

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	Qing You
	Li Suiyan
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Cite this article:

Qing You,Li Suiyan,Weng Jie. Fabrication of Puerarin@FeS₂/Ti₃C₂ Nanoplatform for Postoperative Bone Tumor Therapy[J]. Chinese Journal of Biomedical Engineering, 2026, 45(3): 352-361.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2026.03.010 OR http://cjbme.csbme.org/EN/Y2026/V45/I3/352

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