生物3D打印技术在乳腺癌研究与治疗中的研究进展

doi:10.3969/j.issn.0258-8021.2025.06.011

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Abstract Breast cancer is one of the most common malignant tumors among women worldwide, and its high heterogeneity poses significant challenges for precision therapy in medical research. The 3D bioprinting technology enables precise spatial control of cells, biomaterials, and biomolecules at the microscale, constructing complex tissue structures or microenvironments, thereby providing a novel platform for precision therapy of breast cancer. This paper systematically reviewed recent advancements in 3D bioprinting applications for breast cancer research, with particular focus on its implementation in constructing in vitro tumor models, guiding precision drug therapy, facilitating personalized surgical planning, and post-operative breast reconstruction. Current technical challenges and future development directions were critically discussed. This review aimed to provide valuable references for researchers and clinicians engaged in precision therapy of breast cancer for promoting the clinical translation of 3D bioprinting technology in breast cancer treatment.

Key words： 3D bioprinting breast cancer tumor model precision medicine

Received: 12 April 2025

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R318

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	Articles by authors
	Shi Panpan
	Zhai Weijia
	Wang Xiaofeng
	Zhang Yiqing
	Deng Lili
	Tang Ningning
	Wang Yunlong

Cite this article:

Shi Panpan,Zhai Weijia,Wang Xiaofeng, et al. Research Advances in 3D Bio-printing for Breast Cancer[J]. Chinese Journal of Biomedical Engineering, 2025, 44(6): 748-758.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2025.06.011 OR http://cjbme.csbme.org/EN/Y2025/V44/I6/748

[1] Kim J, Harper A, McCormack V, et al. Global patterns and trends in breast cancer incidence and mortality across 185 countries [J]. Nat Med, 2025;31(4):1154-1162.
[2] Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. [J].CA Cancer J Clin. 2024;74(3):229-263.
[3] Siegel RL, Kratzer TB, Giaquinto AN, et al. Cancer statistics, 2025 [J]. CA Cancer J Clin, 2025,75(1):10-45.
[4] 牟思博,周昌明,郑莹.2025年美国癌症统计数据解读及对中国癌症防治工作的借鉴意义[J].中国癌症杂志,2025,35(6):523-530.
[5] 赫捷,陈万青.中国女性乳腺癌发病与死亡趋势分析(2022)[J].中华肿瘤杂志, 2023, 45(2): 123-130.
[6] Tiwari P, Shukla PR, Yadav K, et al. YIGSR Functionalized hybrid exosomes spatially target dasatinib to laminin receptors for precision therapy in breast cancer[J]. Adv Healthc Mater,2025,14(9):e2402673.
[7] Bianchini G, Balko JM, Mayer IA, et al. Triple-negative breast cancer: challenges and opportunities of a heterogeneous disease[J]. Nat Rev Clin Oncol, 2016, 13(11): 674-690.
[8] Lloyd RM, Jhaveri K, Kalinsky K, et al. Precision therapeutics and emerging strategies for HR-positive metastatic breast cancer[J]. Nat Rev Clin Oncol,2024,(21):743-761.
[9] Junttila MR, Sauvage FJD. Influence of tumour micro-environment heterogeneity on therapeutic response[J]. Nature, 2013, 501(7467): 346-354.
[10] Sabit H, Adel A, Abdelfattah MM, et al. The role of tumor microenvironment and immune cell crosstalk in triple-negative breast cancer (TNBC): emerging therapeutic opportunities[J]. Cancer Lett, 2025,628:217865.
[11] Groll J, Boland T, Blunk T, et al. Biofabrication: reappraising the definition of an evolving field[J]. Biofabrication, 2016, 8(1): 013001.
[12] Callejo GP, Astrain GC, Ruiz HA, et al.3D bioprinted tumor-stroma models of triple-negative breast cancer stem cells for preclinical targeted therapy evaluation[J]. ACS Appl Mater Interfaces, 2024,16(21):27151-27163.
[13] Darbandi RM, Darbandi M, Darbandi S, et al. Artificial intelligence breakthroughs in pioneering early diagnosis and precision treatment of breast cancer: a multimethod study[J]. Eur J Cancer, 2024,209:114227.
[14] Prendergast ME, Burdick JA. Recent advances in enabling technologies in 3D printing for precision medicine[J]. Adv Mater, 2020,32(13):e1902516.
[15] Ozbolat IT, Hospodiuk M. Current advances and future perspectives in extrusion-based bioprinting[J].Biomaterials, 2016, 76: 321-343.
[16] Carley O, Yimai C, Ajinkya G, et al. Bioprintable, stiffness-tunable collagen-alginate microgels for increased throughput 3D cell culture studies[J]. ACS Biomater Sci Eng, 2021,7(6):2814-2822.
[17] Chaudhuri O, Cooper-White J, Janmey PA, et al. Effects of extracellular matrix viscoelasticity on cellular behaviour[J]. Nature, 2020, 584(7822): 535-546.
[18] 季烨,魏朝,李昂,等. 3D打印技术在生物医学领域的应用进展[J].中国医疗设备,2024,39(10):175-180.
[19] Mahdizadeh N, Khorshid Shabestari M, Tafvizi F, et al. Delivery of letrozole-encapsulated niosomes via a 3D bioprinting gelatin-alginate scaffold for potential breast cancer treatment [J]. Cancer Nano, 2024,15(1):33.
[20] Horder H, Böhringer D, Endrizzi N, et al. Cancer cell migration depends on adjacent ASC and adipose spheroids in a 3D bioprinted breast cancer model[J]. Biofabrication,2024,16(3):38934608.
[21] Razzaghi M, Akbari M. 3D printed hollow microneedles: the latest innovation in drug delivery. [J].Expert Opin Drug Deliv, 12 Aug, 2025[Epub ahead of print].
[22] Khanmohammadi M, Volpi M, Walejewska E, et al. Printing of 3D biomimetic structures for the study of bone metastasis: a review[J]. Acta Biomater,2024,178(1):24-40.
[23] Sera H, Myong JS. 3D bioprinted drug-resistant breast cancer spheroids for quantitative in situ evaluation of drug resistance[J]. Acta Biomater. 2022,138:228-239.
[24] Lugovoi EM, Karshieva SS, Usatova SV, et al. The design of the spheroids-based in vitro tumor model determines its biomimetic properties[J]. Biomater Adv, 2025,169:214178.
[25] Monteiro VM, Rocha M, Carvalho TM, et al. Embedded bioprinting of tumor-scale pancreatic cancer-stroma 3D models for preclinical drug screening[J]. ACS Appl Mater Interfaces,2024,16(42): 56718-56729.
[26] Silva DBP, Oliveira R, Araújo M, et al. An integrative alginate-based 3D in vitro model to explore epithelial-stromal cell dynamics in the breast tumor microenvironment[J].Carbohydr Polym, 2024,342:122363.
[27] Dey M, Kim MH, Nagamine M, et al. Biofabrication of 3D breast cancer models for dissecting the cytotoxic response of human T cells expressing engineered MAIT cell receptors[J]. Biofabrication, 2022,14(4):1-27.
[28] Ferreira PL, Jorge C, Pereira HM, et al. Flow-on-repellent biofabrication of fibrous decellularized breast tumor-stroma models[J]. Biomater Adv, 2025;166:214058.
[29] Grigoryan B, Paulsen JS, Corbett CD, et al. Multivascular networks and functional intravascular topologies within biocompatible hydrogels[J]. Science,2019,364(6439):458-464.
[30] Chen Shengnan, Li Yuxuan, Yu Chenggong, et al. 3D bioprinted tumor-vessel-bone co-culture scaffold for breast cancer bone metastasis modeling and drug testing[J]. Chem Eng J,2023,476:146685.
[31] Desigaux T, Comperat L, Dusserre N, et al. 3D bioprinted breast cancer model reveals stroma-mediated modulation of extracellular matrix and radiosensitivity[J]. Bioact Mater, 2024,5(42):316-327.
[32] 黄贝思. 生物3D打印异质肿瘤模型用于药物筛选研究[D].杭州:杭州电子科技大学, 2023.
[33] Dhand AP, Davidson MD, Burdick JA. Lithography-based 3D printing of hydrogels[J]. Nat Rev Bioeng, 2025;3(2):108-125.
[34] Nafiseh M, Ali SH, Burak AD, et al. Controlled tumor heterogeneity in a co-culture system by 3D bio-printed tumor-on-chip model[J]. Sci Rep, 2023;13(1):13648.
[35] Ahn M, Park TG, Shukla KA, et al.3D bioprinting-assisted engineering of stem cell-laden hybrid biopatches with distinct geometric patterns considering the mechanical characteristics of regular and irregular connective tissues[J]. Adv Healthc Mater, 7 July, 2025 [Epub ahead of print].
[36] Revokatova D, Bikmulina P, Heydari Z, et al. Getting blood out of a stone: vascularization via spheroids and organoids in 3D bioprinting. [J]. Cells,2025,14(9):665.
[37] Wang X, Luo Y, Ma Y, et al. Converging bioprinting and organoids to better recapitulate the tumor microenvironment[J]. Trends Biotechnol,2024,42(5):648-663.
[38] Peng Zheng, Lv Sunhao.3D tumor cultures for drug resistance and screening development in clinical applications[J]. Mol Cancer, 2025,24(1):93.
[39] Cui L, Perini G, Minopoli A, et al. Plant-derived extracellular vesicles release combined with systemic DOX exhibits synergistic effects in 3D bioprinted triple-negative breast cancer[J]. Biomed Pharmacother, 2024;181:117637.
[40] Yang Yikun, Qiao Xiaoyin, Huang Ruiying, et al. E-jet 3D printed drug delivery implants to inhibit growth and metastasis of orthotopic breast cancer[J]. Biomaterials, 2020(230): 119618.
[41] Xie Mingjun, Gao Qing, Fu Jianzhou, et al. Bioprinting of novel 3D tumor array chip for drug screening[J]. Bio-Des Manuf,2020,3(3):1-14.
[42] Yuan Shifang, Wei Zhuofan, Hu Bo et al.Visual capturing and profiling of circulating tumor cells from breast cancer patient′s blood via combining fluorescent IMNPs in a magnet-integrated 3D-printed microfluidic chip[J].JOL, 2025, 43(16)suppl:e13038.
[43] Eliana S, Roy F, Yoel G, et al. A fully 3D-printed versatile tumor-on-a-chip allows multi-drug screening and correlation with clinical outcomes for personalized medicine[J]. Commun Biol, 2023,6(1):1157.
[44] Li ST, Chou YH, Huang HJ, et al. Exploring the benefits of 3D-printed bolus in cone beam CT for modified radical mastectomy breast cancer[J]. Int J Radiat Oncol Biol Phys,2023,117(2S):e685.
[45] Wang Jun, Zheng XiangZhong, Tan Chenfeng, et al. Individualized 3D-printed bolus promotes precise postmastectomy radiotherapy in patients receiving breast reconstruction [J]. Front Oncol, 2023,13:1239636.
[46] Wang Yaxue, Li Hanzong, Hu Wendie, et al. The dosimetric value and safety evaluation of 3D printed bolus in adjuvant intensity-modulated radiotherapy after radical mastectomy for breast cancer: a prospective cohort study[J]. Radiat Oncol, 2025;20(1):91.
[47] 成宇,彭海燕,靳富,等.3D打印技术在放疗组织补偿物中的研究进展[J].中国医疗器械杂志,2025,49(2):154-160.
[48] Wang Yong, Huang Fujing, Han Wenmin, et al. Innovative applications of visualized thermosensitive color-changing personalized boluses in post-mastectomy radiotherapy: a dosimetric analysis[J]. Radiat Oncol,2025,20(1):44.
[49] Wang Xiran, Zhao Jianling, Xiang Zhongzhen, et al. 3D-printed bolus ensures the precise postmastectomy chest wall radiation therapy for breast cancer[J]. Front Oncol, 2022, 12:964455.
[50] Lobo D, Srinivas C, Banerjee S, et al. Estimation of surface doses in the presence of an air gap under a bolus for a 6 MV clinical photon be am-a phantom study[J]. Radiat Environ Biophys,2025,64(1):77-83.
[51] McCallum S, Maresse S, Fearns P. Evaluating 3D-printed bolus compared to conventional bolus types used in external beam radiation therapy[J]. Curr Med Imaging, 2021, 17(7):820-831.
[52] 孙涛,由渭平,邓飞,等.混合现实技术远程诊治乳腺肿瘤患者的价值研究[J].中国肿瘤外科杂志,2019,11(3):161-164.
[53] 孙涛,张鹤达,李建,等.个案报道:基于3D打印技术的乳腺肿瘤精准手术治疗2例[J].中国肿瘤外科杂志,2016,8(4):235-239.
[54] Lu Yahui, Chen Xing, Han Fang, et al.3D printing of self-healing personalized liver models for surgical training and preoperative planning[J]. Nat Commun,2023,14(1):8447.
[55] Natividad GJAC, Gomez-Roman N, Chalmers AJ. Patient-specific 3D-printed glioblastomas [J].Nat Biomed Eng, 2019,3(7):498-499.
[56] 张颖,王传兵,张玲,等. 3D打印技术在乳腺肿瘤精确定位中的应用研究[J]. 中国医学装备, 2021, 18(11): 52-55.
[57] Wang Lu, Ye Chenyang Xue Xiangjie, et al.3D-printed breast prosthesis that smartly senses and targets breast cancer relapse[J]. Adv Sci (Weinh),2024;11(46):e2402345.
[58] Zhang Juliang. Application of 3D-printed biodegradable scaffolds in breast reconstruction after mastectomy [C] //2024 San Antonio Breast Cancer Symposium. San Antonio: SABCS, 2024: 4-04-42.
[59] Bai Guo,Yuan Pingyun,Cai Bolei,et al. Stimuli-responsive scaffold for breast cancer treatment combining accurate photothermal therapy and adipose tissue regeneration[J].Adv Funct Mater, 2019,29(36):1904401.
[60] 李霞飞,闫欢欢,杨托,等.3D生物打印技术的分类评价及其应用于组织工程血管构建的研究进展[J].生物工程学报,2024,40(9):2934-2947.
[61] Zhang Zhanyi, Chen Xuebo, Gao Sujie, et al.3D bioprinted tumor model: a prompt and convenient platform for overcoming immunotherapy resistance by recapitulating the tumor microenvironment[J]. Cell Oncol (Dordr),2024,47(3):1113-1126.
[62] Miller IR, Bui H, Wood JB, et al.Understanding phycosomal dynamics to improve industrial microalgae cultivation[J]. Trends Biotechnol,2024,42(6):680-698.
[63] Sahranavard M,Zamanian A, GhaderAB, et al. Optimization of gellan gum-based bioink printability for precision 3D bioprinting in tissue engineering[J]. Int J Biol Macromol. 2025;320(Pt2):145800.
[64] Soufivand AA, Lee JS, Jüngst T, et al. Challenges and perspectives in using finite element modeling to advance 3D bioprinting[J]. Prog Biomed Eng (Bristol), 2025;7(3):032004.
[65] Skylar-Scott MA, Uzel SGM, Nam LL, et al. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels[J]. Sci Adv,2019,5(9):eaaw2459.
[66] 王梓霏,丁雅卉,李彦,等.生物3D打印在肿瘤研究及组织工程中的应用[J].中国癌症杂志,2024,34(9):814-826.
[67] He Chaofan, He Jiankang, Wu Chentie, et al. 3D printing for tissue/organ regeneration in China[J]. Bio-Des Manuf,2025,8(2):169-242.
[68] Vijayavenkataraman S.3D bioprinting: challenges in commercialization and clinical translation[J]. J 3D Print Med,2023,7(2). 3DP8.