电沉积纳米金修饰的16通道电流型PSA免疫传感器的制备

doi:10.3969/j.issn.0258-8021.2015.01.008

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Abstract Numerous studies have been reported about electrochemical immunosensor in recent years, while there still exist some shortcomings like complex preparation processes, low sensitivity, bad specificity and high cost. The purpose of this study is to improve the immunosensor performance using 16 channels screenprinted electrode (16SPCE) modified by gold nanoparticles electrodeposition. The surface of 16SPCE was modified by gold nanoparticles which was reduced by HAuCl 4 through potentiostatic deposition. HAuCl 4 solution with concentration of 005 mg/mL and deposition time of 150 s were the best condition for the 16SPCE modified by gold nanoparticles. Prostate antigen (PSA) antibody was fixed to the electrode surface based on electrostatic adsorption principle and PSA immunity electrode was made using double antibody sandwich method. The electrode performance was evaluated by cyclic voltammetry method and steady state IT curves method. The results of serum PSA levels detected by the immunosensor from 30 prostate cancer patient samples and 3 normal samples were consistent with those of ELISA method. The linear range of the immunesensor is from 0.2 ng/mL to 100 ng/mL, the linear regression equation is Y=134.558X+72.705, the linear correlation coefficient is 1 and the detection limit is 0.14 ng/mL. The results of serum PSA level from prostate cancer patient samples detected by the immunesensor show a good linear correlation with those of ELISA (R=0.944, P<0.001) and the results of serum PSA levels from normal samples are in the range of normal values. It means that the immunesensor based on 16 channel screenprinted electrode modified by gold nanoparticles has the advantages of high sensitivity, good specificity, low cost and short response time. The electrode shows promising clinical setting in the future.

Key words： gold noparticles electrodeposition 16 channel screen printed electrode electrochemical immunosensor prostate specific antigen

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	Wang Zehua¹
	2 Zeng Dongdong²Zhang Huan²Mi Xianqiang^2*

Cite this article:

Wang Zehua^1,2Zeng Dongdong²Zhang Huan²Mi Xianqiang^2*. 16-SPCEAmperometric PSA Immunosensor Modified by Gold Nanoparticles Electrodeposition[J]. journal1, 2015, 34(1): 55-61.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2015.01.008 OR http://cjbme.csbme.org/EN/Y2015/V34/I1/55

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