低强度脉冲超声波联合引导骨再生术对Beagle犬牙周骨开窗缺损修复效应的研究

doi:10.3969/j.issn.0258-8021.2012.06.014

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Abstract The aim of this work is to investigate the effect of combined application of lowintensity pulsed ultrasound (LIPUS) and guided bone regeneration (GBR) on canine periodontal fenestration defect in Beagle dogs. In the experiments, five Beagle dogs were used for establishing 5 mm×5 mm periodontal fenestration defect. Four canines in each dog were randomly divided into LIPUS（90 mW/cm², 20 min/d）group, LIPUS（90 mW/cm², 20 min/d）+ GBR group, GBR group and Control group. Each group had 5 teeth. In order to compare the tissue repair among the groups, the specimens of periodontal fenestration were obtained for histological analysis (HE and Masson staining), Micro-CT test and histometric analysis (the measurement of new bone area, NBA and its percentage in initial defect area, NBA%) after 4 weeks. Micro-CT test results showed that the area of new alveolar bone from large to small was LIPUS+GBR group, GBR group, LIPUS group, and Control group. Histological analysis showed that the Masson staining of new bone in LIPUS and LIPUS+GBR group was mainly red while GBR and control group was mainly blue. It suggested the new bone collagen in LIPUS group and LIPUS+GBR group was more mature than in GBR group and control group. Histometric analysis showed that NBA and NBA% were 0.39 mm² and 7.74% in respectively LIPUS group; 0.52 mm² and 10.30% respectively in LIPUS+GBR group; 0.41 mm² and 7.44% respectively in GBR group; 0.24 mm² and 4.64% respectively in Control group. Differences between groups were significant (P＜0.05). It suggested the amount of new alveolar bone from large to small was LIPUS+GBR group, GBR group, LIPUS group, and Control group. LIPUS combined with guided bone regeneration (GBR) can promote early repair of periodontal fenestration defect.

Key words： low-intensity pulsed ultrasound guided bone regeneration beagle dog periodontal fenestration defect repair

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	GAO Xiang¹SONG Jin Lin^1*DENG Feng¹ZHAO Chun Liang²WANG Zhi Biao^2

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GAO Xiang¹SONG Jin Lin^1*DENG Feng¹ZHAO Chun Liang²WANG Zhi Biao^2. Effect of LowIntensity Pulsed Ultrasound Combined with Guided Bone Regeneration on Canine Periodontal Fenestration Defect in Beagle Dogs[J]. journal1, 2012, 31(6): 895-901.

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http://cjbme.csbme.org/EN/10.3969/j.issn.0258-8021.2012.06.014 OR http://cjbme.csbme.org/EN/Y2012/V31/I6/895

［1］吴鹏，宋锦璘，冯格，等. 低强度脉冲超声波对Beagle犬牙槽骨缺损的修复效应［J］. 华西口腔医学杂志, 2010, 28(5): 522-525.
［2］孟焕新. 牙周病学［M］ (第.3版). 北京：人民卫生出版社, 2008: 264-269.
［3］Keles GC, Sumer M, Cetinkaya BO, et al. Effect of autogenous cortical bone grafting in conjunction with guided tissue regeneration in the treatment of intraosseous periodontal defects ［J］. Eur J Dent, 2010, 4(4): 403-411.
［4］Sato N, 编，王勤涛, 译. 牙周外科学临床图谱［M］. 北京：人民军医出版社, 2005: 133-136.
［5］Becker W, Dahlin C, Becker BE, et al. The use of ePTFE barrier membranes for bone promotion around titanium implants placed into extraction sockets: a prospective multicenter study ［J］. Int J Oral Maxillofac Implants, 1994, 9(1): 31-40
［6］Gher ME, Quintero G, Sandifer JB, et al. Combined dental implant and guided tissue regeneration therapy in humans ［J］. Int J Periodontics Restorative Dent, 1994, 14(4): 332-347
［7］何平，高翔，宋锦璘，等. 低强度脉冲超声波对Beagle犬Ⅱ度根分叉病变的辅助效应［J］. 中国组织工程研究与临床康复, 2011, 15(7): 1219-1223.
［8］董妮，宋锦璘，冯格，等. 低强度脉冲超声波对Beagle犬牙周炎组织修复效应的初步研究［J］. 四川大学学报(医学版), 2012, 43(2): 183-187.
［9］李娜，卢礼，宋锦璘，等. 低强度脉冲超声波联合牙周组织翻瓣移植修复Beagle犬骨上缺损型牙周病［J］. 中国组织工程研究, 2012, 16(5): 766-769.
［10］刘婷，宋锦璘，刘珉懿，等. 低强度脉冲超声波对Beagle犬下颌第三前磨牙慢性牙周炎组织学效应的探讨［J］. 中国超声医学杂, 2011, 27(11): 970-973.
［11］Ikai H, Tamura T, Watanabe T, et al. Lowintensity pulsed ultrasound accelerates periodontal wound healing after flap surgery ［J］. J Periodont Res, 2008, 43(2): 212-216.
［12］杨尊，任蕾西，宋锦璘，等. 低强度脉冲超声刺激对人类牙周膜细胞BMP2表达效应的研究［J］. 中国美容医学, 2011, 20(5): 767-770.
［13］郑鸿，卢礼，宋锦璘，等. 低强度脉冲超声波联合引导组织再生术促进牙周骨开窗缺损修复的动物实验［J］. 中华口腔医学杂志, 2011, 46(7): 431-436.
［14］Kobayashi Y, Sakai D, Iwashina T, et al. Louintensity pulsed ultrasound stimulates cell proliferation, proteoglycan synthesis and expression of growth factorrelated genes in human nuclesus pulposus cell line ［J］. Eur Cell Mater, 2009, 17(3): 15-22.
［15］Zimmerman WK. Periodontal repair in dogs: supraalveolar defect models for evaluation of safety and efficacy of periodontal reconstructive therapy ［J］. Journal of Periodontology, 1994, 65(3): 1151-1157.
［16］Weinberg MA, Bral M. Laboratory animal models in periodontology ［J］. J Clin Periodontol, 1999, 26(6): 335-340. 
［17］HjortingHansen A. Incomplete bone healing of experimental cavities in dog mandibles ［J］. British Journal of Oral Surgery, 1971, 9(1): 33-40. 
［18］Caton J, Motal, Gandinil. Nonhuman primate models for testing the efficacy and safety of periodontal regeneration procedures ［J］. J Periodontol, 1994, 65(12): 1143-1150.
［19］Assaf A. Ridge splitting technique: a 3-D solution for the thin Maxilla ［J］. Dental Horizons. 2006, 2(11): 7-11.
［20］Ming Jiang, Ge Wang, Margaret W, et al. Blind Deblurring of Spiral CT Images. IEEE Trans ［J］. Medical Imaging. 2003, 22(8): 837-845.
［21］Sun Juisheng, Hong RC, Chang W HongShong, et al. In vitro effects of lowintensity ultrasound stimulation on the bone cells ［J］. J Biomed Mater Res, 2001, 57(3): 449-456.
［22］Inubushi T, Tanaka E, Rego EB, et al. Effects of ultrasound on the proliferation and differentiation of cementoblast lineage cells ［J］. J Periodontal. 2008, 79(10): 1984-1990.
［23］郭建刚，赵然，侯桂英，等. 骨组织成分与Masson 三色染色反应的关系分析［J］. 中医正骨, 2001, 13(11): 645-646.