Abstract：The aim of this work is to evaluate the cytotoxicity of titanium dioxide nanoparticles (Nano-TiO2) at different doses based on Cole-Cole model and explore the electrophysiological mechanism. The human gastric cancer cell MGC803 was exposed to 150 mg/L and 300 mg/L Nano-TiO2 suspension for 24 h. The amplitude and phase angle of the impedance of human gastric cancer MGC803 cell suspension were measured by 4294A Agilent precision impedance analyzer in the range of 1 kHz-100 MHz. Based on the residual analysis of the curve fitting of the electrical impedance spectroscopy and the Nyquist plot, the parameters of the Cole-Cole model were established, and the effect of Nano-TiO2 on the conductivity of MGC803 cells was evaluated based on the Cole-Cole model. Results showed that 150 mg/L and 300 mg/L of Nano-TiO2 induced the first impedance increment (ΔZ1) decreased 18.18% (P<0.001) and 39.39% (P<0.001) respectively, the second impedance increment (ΔZ2) decreased 6.56% (P<0.001) and 8.2% (P<0.001) respectively, and reduced the resistance of cellular membrane and nuclear membrane of MGC803 cells, increased its conductivity. The first characteristic frequency (fC1) was increased by 19.74% (P<0.001) and 29.67% (P<0.001) respectively, and second characteristic frequency (fC2) was increased by 6.28% (P<0.001) and 23.43% (P<0.001) respectively. The first dispersion angle (β1) was reduced by 1.35% (P>0.05) and 2.70% (P<0.05), respectively. The Cole-Cole model can be applied to evaluate the role of Nano-TiO2 and explain its electrophysiological mechanism, providing a method for the study of cytotoxicity of nanoparticles.
包秀玲, 廖 聪, 马 青. 基于Cole-Cole模型评价纳米二氧化钛的细胞毒性作用[J]. 中国生物医学工程学报, 2017, 36(3): 336-341.
Bao Xiuling, Liao Cong, Ma Qing. Evaluation of Cytotoxicity of Nano Titanium Dioxide Based on Cole-Cole Model. Chinese Journal of Biomedical Engineering, 2017, 36(3): 336-341.
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