Depthcontrolled and Automated Piezoelectric Micro issection System
1 Jiangsu Provincial Key Laboratory of Advanced Robotics, Suzhou 215021, China
2 Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, China
3 Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Abstract：In order to obtain a specific population of cells or a single cell from different tissue slice for the research, a depthcontrolled and automated piezoelectric microdissection system was developed to solve problems of time consuming, easy fatigue, and low accuracy in the manualdissection. On the basis of different gray values of foreground and background to detect and track the tip of the dissection needle〖DK〗’s position in rea
ltime, and according to the change of the X axis to judge whether the dissection needle contact the target surface, the depth information was acquired. By analyzing the dissection experiments conducted in different conditions, it is shown that this proposed system can place the needle in any positions of the tissues, quickly scan the whole samples to determine the target area and realize the dissection in specify position with arbitrary shape of biological samples. The fineness of cutting line is 15 μm, the resolution of cutting is 50 nm，and cutting error is 05 μm, reaching the unicellular cut level. In addition, the separation of target tissue sections can be realized automatically. The method provides the improvement of the precision and efficiency of dissections, showing its superiority.
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