面向细胞分离的微流控技术

doi:10.3969/j.issn.0258-8021.2013.02.015

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摘要在医学和生物学的多项研究中，能够对不同种类的细胞进行有效的分离，一直是学术界所面临和一直在研究的重要问题。如果人们能够有效地分离不同类型的细胞，那么将会给许多疾病的诊断和治疗带来巨大的便利，从而为医学和生物学带来突破。目前，传统的、主要的细胞分离和筛查方法可以分为两大类，分别是标记法和非标记法。随着微流体技术的发展，微流体芯片正在越来越广泛地应用在细胞分离的领域。本文从微流体的研究历程出发，结合现有的传统细胞分离技术，以及其与微流体技术的对比，对微流体在细胞分离领域的应用和发展作综述性介绍。

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	鲍小凡刘冉^*刘静

关键词 ：细胞分离, 细胞筛选, 微流体, 介电泳, 生物芯片

Abstract：In a number of studies in the field of medicine and biology, it has been an important issue faced and being researched by academia, to separate different kinds of cells effectively. If we can separate different types of cells effectively, it will bring great convenience and benefit to the diagnosis and treatment of many diseases, as well as bring breakthrough to medicine and biology. At present, traditional cell separation and screening　 methods can be divided into two categories: labeling method and nonlabeling method. With the development of microfluidics, microfluidic chip is becoming more and more widely used in the field of cell separation. This paper, starting from the research history and progress of microfluidics, combining with comparison of existing traditional cell separation methods and microfluidic technology, provided a review and introduction of microfluidics usage and development in the field of cell separation.

Key words： cell separation cell screening microfluidics dielectrophoresis biochip

基金资助:国家重大科学仪器设备开发专项（2011YQ030134）；国家自然科学基金 (81071255)；教育部自主科研计划（2012Z01011）

引用本文:

鲍小凡刘冉^*刘静. 面向细胞分离的微流控技术[J]. 中国生物医学工程学报, 2013, 32(2): 226-238.
BAO Xiao Fan LIU Ran^*LIU Jing. Microfluidic Technology for Cell Separation. journal1, 2013, 32(2): 226-238.

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