冻干保护剂和预冻结速率对猪关节软骨压缩杨氏模量的影响

doi:10.3969/j.issn.0258-8021.2013.01.008

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摘要探索冻干保护剂的种类、浓度以及预冻结速率等对猪关节软骨力学性能的影响。采用动态机械热分析仪（DMA）的压缩模式, 研究预冻结速率为1、10、20℃／min，不同体积百分比浓度［10%、20%、30%、40%、50%、60%、80%(V/V）］二甲亚砜（DMSO）、甘油和丙二醇作为保护剂及对猪关节软骨压缩杨氏模量的影响。研究结果表明：1）经中低浓度［小于40%(V/V）］冻干保护剂处理样品的杨氏模量随冻干保护剂浓度的增大而增大，而高浓度范围内［大于40%(V/V）］随冻干保护剂浓度的增大而减小, 如：预冻结速率为1℃／min时，经浓度为10%(V/V）DMSO处理样品的杨氏模量为1.412 MPa，30%(V/V）DMSO为2.031 MPa ，40%(V/V）DMSO为2.331 MPa，50%(V/V）DMSO为2.227 MPa , 60%(V/V）DMSO为1.991 MPa ，80%(V/V）DMSO为1.868 MPa；2）经中低浓度［小于40%(V/V）］冻干保护剂处理的样品采取慢速预冻结速率对于保护冻干关节软骨的杨氏模量效果最好, 如：预冻结速率为1℃／min时，经浓度为40%(V/V）DMSO处理样品的杨氏模量为2.331 MPa, 预冻结速率为10℃／min时，杨氏模量为2.151 MPa；3）在一定浓度范围内［即大于30% (V/V）且小于60%(V/V）］, 相同浓度下样品经DMSO处理的杨氏模量最大，甘油次之，丙二醇的最小。

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	孙慧君胥义^* 吕娅

关键词 ：关节软骨, 动态机械分析仪（DMA）, 杨氏模量, 冻干保护剂, 预冻结速率

Abstract：To explore the effects of cryoprotectants, concentrations and cooling rates on Young’s modulus of porcine articular cartilage (AC), control-force mode of dynamic mechanical analysis (DMA) was used. Cooling rates were set as 1, 10 and 20℃／min. Dimethyl sulphoxide (DMSO), glycerine and 1,2-dihydroxypropane were chosen to be CPAs and the effects of their concentrations ［i.e. 10%, 20% , 30%, 40%, 50%, 60% and 80%(V/V)］ on Young's modulus of porcine articular cartilage were studied. The results showed that: 1) The Young's modulus was increased with the increase of CPA concentration within the low and medium concentration range ［less than 40%(V/V)］. While within high concentration range ［more than 40%(V/V)］ the Young's modulus was decreased with the increasing of CPA concentration. For example, when the cooling rate was 1℃／min, the Young's modulus of sample treated with different DMSO concentrations were 1.412, 2.031, 2.331, 1.991, 2.227 and 1.868 MPa for 10%, 30%, 40%, 50%, 60% and 80%(V/V), respectively. 2) Within the low and medium CPA concentration range, slow cooling rate was beneficial to preserve Young's modulus of AC. For example, when the cooling rate was 1℃／min, the Young's modulus of sample treated with 40%(V/V) DMSO was 2.331 MPa, and 2.151 MPa for 10℃／min. 3) At the concentration range of 30%~60%(V/V), DMSO was the best one to protect the Young’s modulus of AC, glycerin was the second best one, and 1, 2-dihydroxypropan exhibited the lowest performance.

Key words： articular cartilage dynamic mechanical analyzer Young's modulus cryoprotectants cooling rate

引用本文:

孙慧君胥义^* 吕娅. 冻干保护剂和预冻结速率对猪关节软骨压缩杨氏模量的影响[J]. 中国生物医学工程学报, 2013, 32(1): 53-60.
SUN Hui Jun XU Yi^* LV Ya. Effect of Cryoprotectants and Cooling Rates on Young’s Modulus of Porcine Articular Cartilage. journal1, 2013, 32(1): 53-60.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2013.01.008 或 http://cjbme.csbme.org/CN/Y2013/V32/I1/53

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