Abstract：Molecular blotting technology mimics the antigen-antibody specific recognition mechanism of the organism's immune system, and designs and synthesises functional polymers with pre-assembled recognition sites to achieve highly selective recognition and capture of target molecules. In recent years, this technology has demonstrated its potential application in biomedical detection through cross-fertilisation with electrochemical sensing technology, and has attracted much attention for its excellent detection sensitivity, selectivity and stability. In this paper, we systematically review the construction elements of molecularly imprinted electrochemical sensors, focusing on the categories of functional monomers and their selection strategies, as well as the preparation of highly efficient imprinted membranes, and review the progress of the application of such sensors in biomedical detection fields such as biological and clinical marker detection and pathogen identification, and discuss the challenges of the development of sensors in the areas of sensitivity, precision, reproducibility, and suitability for complex biological samples. It also discusses the challenges of sensor development, such as sensitivity, precision, repeatability, and adaptation of complex biological samples, and looks forward to the future development of this field in the application of new smart materials, multimodal sensing integration and standardised detection platform construction, which will provide an important reference to promote the application of molecularly imprinted electrochemical sensors in the field of precision medicine.
师帆, 张国军, 李玉桃, 唐丽娜. 分子印迹电化学传感器的生物医学检测应用:研究进展与挑战[J]. 中国生物医学工程学报, 2025, 44(4): 502-512.
Shi Fan, Zhang Guojun, Li Yutao, Tang Lina. Research Progress on Application of Molecularly Imprinted Electrochemical Sensors in BiomedicalField. Chinese Journal of Biomedical Engineering, 2025, 44(4): 502-512.
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