Research Progress on Application of Isothermal Nucleic Acid Amplification Technology inPoint-of-Care Testing of Pathogens
Wan Jiaxin1,2, Xu Xing2, Xie Chuanqi2, Zhou Xin2, Wang Xingbo2, Zhu Yinchu2, Li Caiyan1, Wu Yue2*, Zhou Weidong2*
1(School of Biology and Environment, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China) 2(Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)
Abstract：With the increasing global public health challenges, the demand for rapid, accurate, and user-friendly pathogen detection technologies has become urgent. Isothermal amplification of nucleic acids (IANA) has emerged as a prominent research focus in the field of pathogenic microorganism detection, owing to its straightforward operational process, minimal equipment requirements, and high detection capabilities. This paper provided a comprehensive review of isothermal nucleic acid amplification-based pathogen detection technologies and their recent advancements in point-of-care testing (POCT). The principles of isothermal nucleic acid amplification, along with its applications in detecting viruses, bacteria, and other pathogenic microorganisms, were emphasized from enzyme-mediated and non-enzyme-mediated methods. Furthermore, the practical applications of POCT devices utilizing isothermal nucleic acid detection technology for pathogen detection are analyzed in detail. Finally, the challenges and future development trends of pathogen detection technology by isothermal nucleic acid amplification were briefly summarized, aiming to provide scientific reference for the development of efficient and rapid detection technology of pathogens.
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