Evaluation of the Safety and Efficacy of Breast Cancer Tumor-Bed Degradable Markers in Rats
Xie Xuejie1,2,3#&, Xie Xin4,5&, Luo Yingen1,2,6, Kong Xiangyi1,2,7, Wang Ningyu1,2,3, Dai Jianrong1,2,3#*
1(National Cancer Center, Beijing 100021, China) 2(National Clinical Research Center for Cancer, Beijing 100021, China) 3 (Department of Radiation Oncology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China) 4(Clinical Oncology School of Fujian Medical University, Fuzhou 350014, China) 5(Department of Radiation Oncology, Fujian Cancer Hospital, Fuzhou 350014, China) 6(Department of Interventional Radiology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China) 7 (Department of Breast Surgery, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China)
Abstract：The aim of this investigation is to evaluate the safety and efficacy of breast cancer tumor-bed biodegradable markers in rats. In experiments, a total of 100 rats were divided into surgical group, titanium clip group and degradable labeling group (n=30), and the other 10 rats were set up for the whole degradable observation group. The biodegradable, buckle-shaped marker with round holes and grooves were made of PLA/β-TCP material, and the markers and titanium clips were implanted into the rat mammary tissue. Hematoxylin and eosin staining (HE) and immunohistochemistry (IHC) were used to analyze the inflammatory responses of rat perimammary tissues after marker implantation. Using CT and MR imaging, the degradable markers and titanium clips were observed for the presence of artifacts, and the ability of the degradable markers to achieve effective visualization over the observation period (46 weeks) was evaluated. Safety results showed that the acute immune responses in the tissues around the degradable markers were higher than that of the titanium clips and returned to normal after 12 weeks. The gap between the expression of IL-6 in the degradable labeling group and the titanium clips group was reduced from 12% to 2.8%. The gap between the expression of IL-10 in the degradable labeling group and the titanium clips group was reduced from 14.8% to 8.1%. Statistical analysis showed that the difference in the rate of positive cells for inflammatory factors was significant in the first 4 weeks. The validity results showed that the boundaries of the degradable markers were clear without obvious artifacts on CT and MR images, and the CT values tended to decrease gradually with the increase of time. The results showed that the inflammatory reaction induced by the degradable marker in rats basically disappeared at week 12, and the imaging effect on CT and MR was better than that of the titanium clip marker. In the future, this new biodegradable marker could potentially be an alternative to titanium clips.
作者简介: #中国生物医学工程学会会员(Member, Chinese Society of Biomedical Engineering) &共同第一作者(Co-first author)
引用本文:
谢雪洁, 谢欣, 骆银根, 孔祥溢, 王宁宇, 戴建荣. 乳腺癌瘤床可降解标记物在大鼠体内的安全性和有效性[J]. 中国生物医学工程学报, 2025, 44(2): 203-210.
Xie Xuejie, Xie Xin, Luo Yingen, Kong Xiangyi, Wang Ningyu, Dai Jianrong. Evaluation of the Safety and Efficacy of Breast Cancer Tumor-Bed Degradable Markers in Rats. Chinese Journal of Biomedical Engineering, 2025, 44(2): 203-210.
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