摘要抑郁症已成为危害人类健康的一大公共卫生问题。目前,抑郁症的诊断主要依靠患者自述或填写专业量表,由医生进行判断。此方法存在误诊率高、一致性差等缺陷。由此,寻找一种精准、高效、便捷的抑郁症生物标志物具有极重要的价值和意义。本研究通过对比抑郁组和对照组大鼠听觉初级皮层处40 Hz听觉稳态响应(ASSR)差异,探究ASSR作为抑郁症诊断靶标的可行性。将24只大鼠随机分为抑郁模型组(n=12)和正常对照组(n=12),模型组通过持续8周的慢性不可预知温和应激刺激(CUMS)进行建模。通过采集并对比两组大鼠在建模前后的糖水偏好、强迫游泳、旷场实验等3种行为学指标,判断建模是否成功。随后进行电生理实验,采集大鼠在40 Hz-click声音刺激下,左右两侧初级听觉皮层处的局部场电位信号(LFPs),并对比试次间相位一致性(ITPC)及诱发功率两种常用指标。结果表明,与正常对照组相比,8周抑郁建模显著降低了模型组糖水偏好率(71.89±6.32 vs 87.65±3.54,P＜0.05)和旷场运动距离(2 219±532 vs 2 930±315,P＜0.05),显著提升了模型组游泳静止时间[(31.53±5.31)s vs (96.18±13.16)s,P＜0.05],并且两组大鼠之间行为学的后测结果同样存在显著差异(P＜0.05),即建立起有效的抑郁模型;电生理结果表明,在click声音下,模型组的两侧初级听觉皮层40 Hz-ASSR的ITPC值均低于0.6,显著低于对照组(ITPC＞0.8)(P＜0.05),且诱发功率也呈现降低趋势。Click声音诱发的初级听觉皮层处40 Hz-ASSR有希望作为抑郁症的潜在诊断靶标,此发现为辅助诊断和治疗抑郁症提供了一定参考。
Abstract：Depression has become a major public health problem endangering human health. At present, the diagnosis of depression mainly depends on patients' self-reports or filling in professional scales, which are judged by doctors and has the disadvantages of high misdiagnosis rate and poor consistency. Therefore, it is of great value and significance to find an accurate, efficient and convenient biomarker of depression. By comparing the difference of 40 Hz auditory steady-state response (ASSR) in the primary auditory cortex between the depression group and the control group, this study explored the feasibility of ASSR as a diagnostic target of depression, in order to provide ideas for the diagnosis and treatment of depression. In this work, 24 rats were randomly divided into depression model group (n=12) and normal control group (n=12). The model group was established by chronic unpredictable mild stress stimulation (CUMS) lasting for 8 weeks. By collecting and comparing three behavioral indicators of the two groups of rats before and after modeling, such as sugar water preference, forced swimming, open field experiment, we could judge whether the modeling was successful. Then, electrophysiological experiments were carried out to collect local field potential signals (LFPs) at the left and right primary auditory cortex of rats under 40 Hz click sound stimulation. The results showed that the 8-week depression modeling significantly reduced the sugar water preference rate(71.89±6.32 vs 87.65±3.54,P＜0.05)and open field movement distance of the model group(2 219±532 vs 2 930±315,P＜0.05), significantly increased the swimming rest time of the model group [ (31.53±5.31)s vs (96.18±13.16)s,P＜0.05], and there was also a significant difference in the behavioral post-test results between the two groups (P<0.05), which indicated an effective depression model had been established. Electrophysiological results showed that under click sound, the ITPC value of 40 Hz ASSR in both primary auditory cortex of the model group was lower than 0.6, significantly lower than that of the control group (ITPC > 0.8) (P < 0.05), and the evoked power also showed a downward trend. Based on the above results, this study found out that 40 Hz ASSR in the primary auditory cortex induced by click sound could be a potential diagnostic target for depression, which provided a certain reference for the auxiliary diagnosis and treatment of depression.
任政羽, 何雨晨, 刘爽, 刘潇雅, 柯余峰, 陈龙, 明东. 抑郁大鼠40 Hz听觉稳态响应的特性研究[J]. 中国生物医学工程学报, 2023, 42(1): 1-9.
Ren Zhengyu, He Yuchen, Liu Shuang, Liu Xiaoya, Ke Yufeng, Chen Long, Ming Dong. Study on Characteristics of 40 Hz-ASSR in Depressed Rats. Chinese Journal of Biomedical Engineering, 2023, 42(1): 1-9.
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