基于少层MoS<sub>2</sub>纳米片的光学生物传感器用于癌症标志物ctDNA的检测

doi:10.3969/j.issn.0258-8021.2024.02.006

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摘要癌症已成为威胁人类健康和生命的主要疾病之一。循环肿瘤DNA(ctDNA)检测作为一种实用的液体活检技术,在肿瘤诊断、靶向治疗和预后预测中具有重要的应用前景。因此,本课题研究了一种基于少层MoS₂纳米片的光学生物传感器用于癌症标志物ctDNA的高灵敏检测。首先,采用剪切剥离法制备少层MoS₂纳米片,并对制备条件进行优化,以制备横向尺寸较大、厚度均匀的少层MoS₂纳米片;其次,通过分散液吸光度的变化研究少层MoS₂纳米片在高浓度盐溶液中的加速聚集沉淀行为;然后,基于MoS₂纳米片对单链DNA的吸附力,研究了单链DNA对于盐诱导沉淀少层MoS₂纳米片的抑制作用;最后,利用杂交形成的双链DNA从少层MoS₂纳米片上脱落而导致的分散液吸光度的变化构建光学生物传感器,并通过cpDNA与不同浓度的ctDNA杂交对该传感器的性能进行检测。结果表明,当cpDNA 浓度为100 nM 时,浓度范围在25 ~ 100 nM的ctDNA与少层MoS₂纳米片分散液的吸光度呈反比线性关系。在401和448 nm波长处时,少层MoS₂纳米片分散液的吸光度与ctDNA浓度的线性回归方程分别为Y=0.235 57-0.000 70X,R²=0.942 22和Y=0.212 53-0.000 50X,R²=0.951 41。所构建的光学生物传感器成功地实现了对癌症标志物ctDNA的检测;为未来的体外癌症检测和诊断提供了一种有效的传感策略。

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	卫瑚玥
	李杜娟
	崔治莲
	樊凯
	杨伟煌
	刘红英
	李丽丽
	吴薇
	王高峰

关键词 ：少层MoS₂纳米片, ctDNA, 生物传感器, 紫外光谱

Abstract：Cancer has become one of the major diseases threatening human health and life. Circulating tumor DNA (ctDNA) testing, as a practical liquid biopsy technique, is a promising method for cancer diagnosis, targeted therapy and prognosis. In this work, an optical biosensor based on few-layer MoS₂ nanosheets was studied for the highly sensitive detection of tumor marker ctDNA. Firstly, The few-layer MoS₂ nanosheets were prepared by shear exfoliation method, and the preparation conditions were optimized to prepare the few-layer MoS₂ nanosheets with large transverse size and uniform thickness. Secondly, the accelerated aggregation and precipitation behavior of MoS₂ nanosheets in high concentration salt solution was studied by detecting the absorbance change of dispersion. Then, based on the adsorption capacity of MoS₂ nanosheets on single-stranded DNA, the inhibition effect of single-stranded DNA on salt-induced precipitation of few-layer MoS₂ nanosheets was studied. Finally, the optical biosensor was constructed by using the change of absorbance of dispersion caused by the fall off of double-stranded DNA formed by hybridization from the few-layer MoS₂ nanosheets, and the performance of the sensor was tested by hybridizing cpDNA with different concentrations of ctDNA. Results showed that the absorbance of ctDNA in the concentration range of 25 nM - 100 nM was inversely linear with the dispersion of MoS₂ nanosheets when cpDNA concentration was 100 nM. The linear regression equations between the absorbance of the dispersion of few-layer MoS₂ nanosheets and the ctDNA concentration at wavelengths of 401 nm and 448 nm were Y=0.235 57-0.000 70X, R²=0.942 22 and Y=0.212 53-0.000 50X, R²=0.951 41, respectively. This study demonstrated that the optical biosensor was able to detect tumor marker ctDNA and provided an effective sensing strategy for future in vitro cancer detection and diagnosis.

Key words： few-layer MoS₂ nanosheets ctDNA biosensor ultraviolet spectrum

收稿日期: 2022-12-01

PACS:

R318

基金资助:浙江省自然科学基金(LY24F010008);国家自然科学基金重大研究计划重点项目(92373202);国家自然科学基金专项研究重点项目(62141409)

通讯作者: ^* E-mail: dujuanli@hdu.edu.cn

作者简介: ^#中国生物医学工程学会高级会员

引用本文:

卫瑚玥, 李杜娟, 崔治莲, 樊凯, 杨伟煌, 刘红英, 李丽丽, 吴薇, 王高峰. 基于少层MoS₂纳米片的光学生物传感器用于癌症标志物ctDNA的检测[J]. 中国生物医学工程学报, 2024, 43(2): 183-195.
Wei Huyue, Li Dujuan, Cui Zhilian, Fan Kai, Yang Weihuang, Liu Hongying, Li Lili, Wu Wei, Wang Gaofeng. An Optical Biosensor Based on Few-Layer MoS₂ Nanosheets for Sensitive Detection of TumorMarker ctDNA. Chinese Journal of Biomedical Engineering, 2024, 43(2): 183-195.

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http://cjbme.csbme.org/CN/10.3969/j.issn.0258-8021.2024.02.006 或 http://cjbme.csbme.org/CN/Y2024/V43/I2/183

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