Study on the Mechanism of Tannic Acid Inducing Bovine Jugular Vein Cross-Linking
Zheng Xiaobing1, Zhou Jianye1*, Wang De1, Dai Zehui2, Guo Baohua2
1(State Key Laboratory of Cardiovascular Disease, Fu Wai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China) 2(Key Laboratory of Advanced Materials, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100037, China)
Abstract：The aim of this study was to investigate chemical reactions of tannic acid (TA) with bovine jugular vein (BJV). Glutaraldehyde-fixed BJVs were subsequently treated with TA or four substitutional compounds, which contained four characteristic chemical groups of tannic acid. The treated BJVs were implanted subcutaneously into 20 SD rats for 21 and 60 days. The calcium content of the explanted tissues was assessed using atomic absorption spectrometry, and the degree of cross-linking was measured by Masson and EVG staining. In vitro, they were characterized with thermal stability, resistance to enzymatic hydrolysis, mechanical properties. The Fourier transform infrared spectroscopy (FTIS) was employed to inspect the changes of absorption curve. Furthermore, a gradient concentration of urea (0.1, 0.3, 0.5 and 1 M) was used to verify if hydrogen bond was formed. Tannic acid treated BJV, with polyphenolic hydroxyl groups in the compound, showed the best performance among the all BJVs. Which suggested effective cross-linking was formed in the tissue, and the calcium content at 21 d and 60 d was 2.25 mg/g and 8.26 mg/g respectively (P< 0.05). FTIS results indicated that hydrogen bond was formed in TA group and it was confirmed by urea test. The urea test showed that the effect of cross-linking between tissue decreased (P< 0.05) with the increase of urea concentration, and the calcium content of TA group was 8.10 mg/g at 60 d, which was much lower than 16.83 mg/g of the 0.1 M urea group and 50.76 mg/g of the 0.5 M urea group(P < 0.001). Our results demonstrated that the hydrogen bond was formed between polyphenolic hydroxyl groups and proteins in TA treated BJVs. It may provide a clue to improve the stability of the reaction system.
郑晓冰, 周建业, 王德, 戴泽辉, 郭宝华. 单宁酸与牛颈静脉的交联机制研究[J]. 中国生物医学工程学报, 2019, 38(6): 733-741.
Zheng Xiaobing, Zhou Jianye, Wang De, Dai Zehui, Guo Baohua. Study on the Mechanism of Tannic Acid Inducing Bovine Jugular Vein Cross-Linking. Chinese Journal of Biomedical Engineering, 2019, 38(6): 733-741.
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