Abstract：In this work, four kinds of chitosan/poly hydroxybutyrate-co-hydroxyvalerate(PHBV)composite scaffold were fabricated by the method of two phase mixture freeze drying.The repair effects of the scaffoldswere investigated and compared to determine the optimal formula for guiding bone regeneration as well as drug releasing. The icariin (Ica) was used in this study as an osteoinductive factor, and Ica microspheres were fabricated by W/O method and covalent binding. Microstructures of the scaffolds and drug releasing behaviors were investigated.After that,the scaffolds were implanted in the radius defects of rabbits;X-ray and 3D CT were applied to observe the repair effect at 1, 3, and 6 months post surgery. The osteogenesis inducible effects were evaluated using HE and Masson staining. The scaffolds showed network-like microstructures with particles inside, the drug loaded particles was 3-11 μm in diameter. The scaffolds released Ica well, though the peak of drug release curve for the covalent binding group was delayed than the others. The peak appeared at 72 h after immersion and quickly reached a stabled level of 75 μg. The images of X-ray and 3-D CT showed the defect of the rabbit radius had connected and the bone mineral density was higher than the other three groups. HE and Masson stained sections of the radius segmental defect of rabbits implanted for 1, 3, and 6 months showed that the scaffold with covalently binding Ica had better repair effect than the others.
李根 甄平 成满平 赵红斌. 用复合诱导型骨修复材料修复兔桡骨缺损的实验研究[J]. 中国生物医学工程学报, 2016, 35(6): 719-728.
Li Gen Zhen Ping Cheng Manping Zhao Hongbin. An Inducible Bone Repair Composite Material PHBV/CS: The Renovation of Rabbit Radius. Chinese Journal of Biomedical Engineering, 2016, 35(6): 719-728.
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